Ents and their tumor tissues differ broadly. Age, ethnicity, stage, histology, molecular subtype, and treatment history are variables that will impact miRNA expression.Table four miRNA signatures for prognosis and remedy response in HeR+ breast cancer subtypesmiRNA(s) miR21 Patient cohort 32 Stage iii HeR2 situations (eR+ [56.two ] vs eR- [43.8 ]) 127 HeR2+ instances (eR+ [56 ] vs eR- [44 ]; LN- [40 ] vs LN+ [60 ]; M0 [84 ] vs M1 [16 ]) with neoadjuvant treatment (trastuzumab [50 ] vs lapatinib [50 ]) 29 HeR2+ situations (eR+ [44.eight ] vs eR- [55.two ]; LN- [34.4 ] vs LN+ [65.six ]; with neoadjuvant therapy (trastuzumab + chemotherapy)+RG7440 price Sample Frozen tissues (pre and postneoadjuvant remedy) Serum (pre and postneoadjuvant remedy)Methodology TaqMan qRTPCR (Thermo Fisher Scientific) TaqMan qRTPCR (Thermo Fisher Scientific)Clinical observation(s) Greater levels correlate with poor remedy response. No correlation with pathologic total response. High levels of miR21 correlate with all round survival. Larger circulating levels correlate with pathologic complete response, tumor presence, and LN+ status.ReferencemiR21, miR210, miRmiRPlasma (pre and postneoadjuvant remedy)TaqMan qRTPCR (Thermo Fisher Scientific)Abbreviations: eR, estrogen receptor; HeR2, human eGFlike receptor 2; miRNA, microRNA; LN, lymph node status; qRTPCR, quantitative realtime polymerase chain reaction.submit your manuscript | www.dovepress.comBreast Cancer: Targets and Therapy 2015:DovepressDovepressmicroRNAs in breast cancerTable five miRNA signatures for prognosis and treatment response in TNBC subtypemiRNA(s) miR10b, miR-21, miR122a, miR145, miR205, miR-210 miR10b5p, miR-21-3p, miR315p, miR125b5p, miR130a3p, miR-155-5p, miR181a5p, miR181b5p, miR1835p, miR1955p, miR451a miR16, miR125b, miR-155, miR374a miR-21 Patient cohort 49 TNBC circumstances Sample FFPe journal.pone.0169185 tissues Fresh tissues Methodology SYBR green qRTPCR (Qiagen Nv) SYBR green qRTPCR (Takara Bio inc.) Clinical observation(s) Correlates with shorter diseasefree and overall survival. Separates TNBC tissues from typical breast tissue. Signature enriched for miRNAs involved in chemoresistance. Correlates with shorter all round survival. Correlates with shorter recurrencefree survival. Higher levels in stroma compartment correlate with shorter recurrencefree and jir.2014.0227 breast cancer pecific survival. purchase RG-7604 Divides situations into danger subgroups. Correlates with shorter recurrencefree survival. Predicts response to treatment. Reference15 TNBC casesmiR27a, miR30e, miR-155, miR493 miR27b, miR150, miR342 miR190a, miR200b3p, miR5125p173 TNBC situations (LN- [35.eight ] vs LN+ [64.2 ]) 72 TNBC cases (Stage i i [45.8 ] vs Stage iii v [54.two ]; LN- [51.three ] vs LN+ [48.6 ]) 105 earlystage TNBC cases (Stage i [48.5 ] vs Stage ii [51.five ]; LN- [67.six ] vs LN+ [32.4 ]) 173 TNBC cases (LN- [35.eight ] vs LN+ [64.two ]) 37 TNBC instances eleven TNBC instances (Stage i i [36.3 ] vs Stage iii v [63.7 ]; LN- [27.two ] vs LN+ [72.eight ]) treated with unique neoadjuvant chemotherapy regimens 39 TNBC circumstances (Stage i i [80 ] vs Stage iii v [20 ]; LN- [44 ] vs LN+ [56 ]) 32 TNBC situations (LN- [50 ] vs LN+ [50 ]) 114 earlystage eR- circumstances with LN- status 58 TNBC circumstances (LN- [68.9 ] vs LN+ [29.three ])FFPe tissues Frozen tissues FFPe tissue cores FFPe tissues Frozen tissues Tissue core biopsiesNanoString nCounter SYBR green qRTPCR (Thermo Fisher Scientific) in situ hybridization165NanoString nCounter illumina miRNA arrays SYBR green qRTPCR (exiqon)84 67miR34bFFPe tissues FFPe tissues FFPe tissues Frozen tissues Frozen tissuesmi.Ents and their tumor tissues differ broadly. Age, ethnicity, stage, histology, molecular subtype, and treatment history are variables which can influence miRNA expression.Table four miRNA signatures for prognosis and treatment response in HeR+ breast cancer subtypesmiRNA(s) miR21 Patient cohort 32 Stage iii HeR2 situations (eR+ [56.2 ] vs eR- [43.8 ]) 127 HeR2+ instances (eR+ [56 ] vs eR- [44 ]; LN- [40 ] vs LN+ [60 ]; M0 [84 ] vs M1 [16 ]) with neoadjuvant therapy (trastuzumab [50 ] vs lapatinib [50 ]) 29 HeR2+ instances (eR+ [44.eight ] vs eR- [55.two ]; LN- [34.four ] vs LN+ [65.6 ]; with neoadjuvant therapy (trastuzumab + chemotherapy)+Sample Frozen tissues (pre and postneoadjuvant therapy) Serum (pre and postneoadjuvant remedy)Methodology TaqMan qRTPCR (Thermo Fisher Scientific) TaqMan qRTPCR (Thermo Fisher Scientific)Clinical observation(s) Greater levels correlate with poor treatment response. No correlation with pathologic total response. High levels of miR21 correlate with overall survival. Greater circulating levels correlate with pathologic comprehensive response, tumor presence, and LN+ status.ReferencemiR21, miR210, miRmiRPlasma (pre and postneoadjuvant therapy)TaqMan qRTPCR (Thermo Fisher Scientific)Abbreviations: eR, estrogen receptor; HeR2, human eGFlike receptor 2; miRNA, microRNA; LN, lymph node status; qRTPCR, quantitative realtime polymerase chain reaction.submit your manuscript | www.dovepress.comBreast Cancer: Targets and Therapy 2015:DovepressDovepressmicroRNAs in breast cancerTable 5 miRNA signatures for prognosis and treatment response in TNBC subtypemiRNA(s) miR10b, miR-21, miR122a, miR145, miR205, miR-210 miR10b5p, miR-21-3p, miR315p, miR125b5p, miR130a3p, miR-155-5p, miR181a5p, miR181b5p, miR1835p, miR1955p, miR451a miR16, miR125b, miR-155, miR374a miR-21 Patient cohort 49 TNBC cases Sample FFPe journal.pone.0169185 tissues Fresh tissues Methodology SYBR green qRTPCR (Qiagen Nv) SYBR green qRTPCR (Takara Bio inc.) Clinical observation(s) Correlates with shorter diseasefree and overall survival. Separates TNBC tissues from standard breast tissue. Signature enriched for miRNAs involved in chemoresistance. Correlates with shorter all round survival. Correlates with shorter recurrencefree survival. Higher levels in stroma compartment correlate with shorter recurrencefree and jir.2014.0227 breast cancer pecific survival. Divides instances into threat subgroups. Correlates with shorter recurrencefree survival. Predicts response to remedy. Reference15 TNBC casesmiR27a, miR30e, miR-155, miR493 miR27b, miR150, miR342 miR190a, miR200b3p, miR5125p173 TNBC situations (LN- [35.8 ] vs LN+ [64.2 ]) 72 TNBC situations (Stage i i [45.8 ] vs Stage iii v [54.two ]; LN- [51.three ] vs LN+ [48.6 ]) 105 earlystage TNBC cases (Stage i [48.five ] vs Stage ii [51.5 ]; LN- [67.6 ] vs LN+ [32.4 ]) 173 TNBC instances (LN- [35.8 ] vs LN+ [64.2 ]) 37 TNBC cases eleven TNBC situations (Stage i i [36.three ] vs Stage iii v [63.7 ]; LN- [27.2 ] vs LN+ [72.eight ]) treated with unique neoadjuvant chemotherapy regimens 39 TNBC cases (Stage i i [80 ] vs Stage iii v [20 ]; LN- [44 ] vs LN+ [56 ]) 32 TNBC circumstances (LN- [50 ] vs LN+ [50 ]) 114 earlystage eR- cases with LN- status 58 TNBC situations (LN- [68.9 ] vs LN+ [29.three ])FFPe tissues Frozen tissues FFPe tissue cores FFPe tissues Frozen tissues Tissue core biopsiesNanoString nCounter SYBR green qRTPCR (Thermo Fisher Scientific) in situ hybridization165NanoString nCounter illumina miRNA arrays SYBR green qRTPCR (exiqon)84 67miR34bFFPe tissues FFPe tissues FFPe tissues Frozen tissues Frozen tissuesmi.
