dly rectifying potassium channels, especially Kir Muller cells where these proteins are particularly enriched in the vitread endfeet and in cell processes abutting the intraretinal blood vessels. Proper functioning of Kir October Homeostasis Regulation by Dp many instances of retinal injuries, including transient ischemia/ reperfusion, diabetic retinopathy, retinal vein occlusion, and retinal detachment. Moreover, the early mislocation of Kir Results Experimental Retinal Detachment induces reactive gliosis and alterations in protein expression in Muller cells dramatic reduction of DpOctober Homeostasis Regulation by Dp Retinal Detachment Causes Morphological and Physiological Alterations in CMuller cells express the inwardly rectifying K+ channel Kir channels on the whole Muller cell is not different between wt and DpOctober Homeostasis Regulation by Dp Other parameters of Muller cell electrophysiology did not show significant differences between control and detached retinas from both strains of mice. Moreover, the application of Ba DpThere were two previous observations which prompted us to study the effect of DpOctober Homeostasis Regulation by Dp. Second, Dp Discussion surrounding blood vessels. It is thus conceivable that the lack of DpOctober Homeostasis Regulation by Dp In conclusion we show here that depletion of Dp alterations. Finally we show the altered Muller cell properties in Dp Materials and Methods Ethics Statement All animal procedures were conducted in accord with UPMC University and INSERM Institutional Animal Care Committee guidelines. Surgery was carried out and/or directly supervised by persons with appropriate levels of experience and training, and surgery performed on animals that will survive was undertaken with careful attention to aseptic technique and prevention of infection. All surgical procedures were completed under anesthesia that renders the animal insensitive to pain. Animals The Dp Antibodies Monoclonal antibodies targeting b-Actin and GFAP were purchased from Sigma-Aldrich and monoclonal antibodies directed against b-dystroglycan were purchased from Novocastra Laboratories. Polyclonal antibodies directed against dystrophins and utrophin were Homeostasis Regulation by Dp previously characterized, whereas the ones directed against Kir amount of Dp Induction of retinal detachment Local retinal detachment 
was induced in the right eye of adult mice, as previously reported with minor modification. Briefly, mice were anesthetized by peritoneal injection of a mixture of Glial cell isolation The retinae were isolated and special care was taken to separate detached areas. Retinal pieces were incubated in papain containing Ca Immunohistochemistry Enucleated eyes were dissected to remove lens and cornea, and fixed by immersion in Electrophysiological recordings Membrane currents of acutely isolated glial cells were recorded in the whole-cell configuration of the patch-clamp technique. Voltageclamp records were performed at room temperature using the Axopatch Western blot BIX01294 web analysis Western blot analysis was performed as previously described. In brief, retinal protein extracts were resolved using NuPAGE TrisAcetate Quantitative RT-PCR analysis of retinal RNA Muller cell swelling To determine volume changes of Muller glial cells evoked by hypotonic stress, the cross-sectional area of glial cell somata in the inner nuclear layer of retinal slices was measured. Acutely isolated slices were prepared from untreated contr
