Rabbit anti-FGFR4 Polyclonal Antibody(N-term)

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description Purified Rabbit Polyclonal Antibody (Pab) |Synonym Fibroblast growth factor receptor 4; FGFR-4; CD334; FGFR4; JTK2; TKF |Host Rabbit |Specificity This FGFR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human FGFR4. |Species Reactivity Human, Rat, Mouse |Applications WB: 1:1000, IHC-P: 1:100-500, FC: 1:10-50 |Properties |Clonality Polyclonal Antibody |Isotype Rabbit Ig |Storage Temp. Maintain refrigerated at 2-8 ° C for up to 2 weeksFor long term storage store at -20 ° C in small aliquots to prevent free that cycles |Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |Research areas Cancer;Cardiovascular;Cell Biology;Neuroscience;Signal Transduction |Target |Background Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. |Cellular localization Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum. Note=Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane |UniProt P22455 |References |References References for protein:1.Jezequel, P., et al., Br. J. Cancer 90(1):189-193 (2004). 2.Qian, Z.R., et al., J. Clin. Endocrinol. Metab. 89(4):1904-1911 (2004). 3.Yu, S., et al., J. Biol. Chem. 278(22):19597-19602 (2003). 4.Becker, N., et al., Cancer Epidemiol. Biomarkers Prev. 12(6):582-583 (2003). 5.Takenaka, H., et al., Arch. Dermatol. Res. 294(7):331-338 (2002).References for HeLa cell line:1. Scherer WF, Syverton JT, Gey GO (May 1953). “Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix”. J. Exp. Med. 97 (5): 695–710. [PubMed:13052828].2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). “Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping”. Cancer Res. 59 (1): 141–50. [PubMed: 9892199]. 3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). “A novel L1 retrotransposon marker for HeLa cell line identification”. BioTechniques 46 (4): 277–84. [PubMed: 19450234].4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). “Check your cultures! A list of cross-contaminated or misidentified cell lines”. Int. J. Cancer 127 (1): 1–8. [PubMed:20143388]. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.