Rabbit anti-pan Cytokeratin Polyclonal Antibody

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Product Details FAQ Citations(0) Video Pictures Documents |Overview |Synonym pan-cytokeratin; pan-CK; pan CK; P-CK; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 1; 2; 4; 5; 6; 7; 8; 71; 72; 75; 78] |Host Rabbit |Specificity Pan Cytokeratin |Species Reactivity Human, Mouse, Rat, Cow |Predicted Reactivity Chicken, Dog, Pig, Horse, Rabbit |Applications WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg /test |Immunogen KLH conjugated synthetic peptide derived from human cytokeratins 1,2,4,5,6,7,8,71,72,75,78: |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Tumor & have spent Cell biology Immunology |Target |Background Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes.
In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection. |Cellular localization Cytoplasm; |UniProt N/A | Sample:Bladder (Mouse) Lysate at 40 ugPrimary: Anti-Pan Cytokeratin (abs123684) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42-64 kDObserved band size: 60 kD| Blank control (blue line): Hela (blue). Primary antibody: Rabbit Anti-Pan Cytokeratin antibody Dilution: 1μg /10^6 cells; Isotype Control antibody: Rabbit IgG . Secondary antibody: Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |Tips:This product is for research use only. Not for use in diagnostic prodcedures.