Product Name :
[pSer78]HSP27 (human), ELISA kit
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Store all components at 4°C, except standard at -20°C.
Description:
Most comprehensive portfolio of heat shock protein (chaperone) products commercially available. Ultra-sensitive and specific – be confident with your results and use less sample per testQuantitative – obtain statistically significant results compared to semi-quantitative Western blot analysisHigher throughput – assay up to 40 samples in duplicate in just 3 hours The [pSer78]HSP27 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.{{878385-84-3} MedChemExpress|{878385-84-3} Protocol|{878385-84-3} References|{878385-84-3} manufacturer} Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes αB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-α, p70 S6K, PKD I, and PKC-δ;. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments.{{1903008-80-9} medchemexpress|{1903008-80-9} Purity & Documentation|{1903008-80-9} Purity|{1903008-80-9} manufacturer} Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation.PMID:31194359 Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.
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| Alternative Name Heat shock protein 27 | Application Colorimetric detection, ELISA | Application Notes For the quantitative determination of human [pSer78]HSP27 in cell lysates, plasma, and serum. | Assay Time 3 hours | Contents Microtiter plate, Conjugate, Antibody, Assay buffer 27, Wash buffer concentrate, Standard, TMB Substrate, 5X Extraction reagent, Stop solution 2 | Crossreactivity Phospho HSP27 (Ser78) (100%), HSP27 (non-phospho) (0.1%) and | GenBank ID L39370 | Sensitivity 4.30 pg/ml (range 31.25 – 1000 pg/ml) | Species Reactivity Human | UniProt ID P04792 | Wavelength 450 nm
