Name :
Human MICA Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
MICA is the highly polymorphic major histocompatability complex class I chain-related protein A. MICA is a single pass type I transmembrane glycoprotein that belongs to the MIC subfamily of MHC-I family of Immunoglobulin superfamily (IgSF). MICA contains 1 Ig-like C1-type motifs (1 IgC1) in the extracellular domain (ECD) in addition to a transmembrane (TM) domain and a cytoplasmic tail. MICA is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. MICA acts as a ligand for the KLRK1/NKG2-D type II integral membrane protein receptor. Binding to KLRK1 leads to cell lysis. MICA also functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma (MM).
Gene Symbol :
NCBI Gene ID :
100507436
Uniprot Entry :
Q29983
Construct Details :
The recombinant human MICA-Fc fusion is expressed as a 511 amino acid protein consisting of Glu24 – His306 region of MICA and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human MICA-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EPHSLRYNLT VLSWDGSVQS GFLTEVHLDG QPFLRCDRQK CRAKPQGQWA EDVLGNKTWD 70 80 90 100 110 120 RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ 130 140 150 160 170 180 NLETKEWTMP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLKSGVVLRR 190 200 210 220 230 240 TVPPMVNVTR SEASEGNITV TCRASGFYPW NITLSWRQDG VSLSHDTQQW GDVLPDGNGT 250 260 270 280 290 300 YQTWVATRIC QGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHVDKTHTC PPCPAPELLG 310 320 330 340 350 360 GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY 370 380 390 400 410 420 NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE 430 440 450 460 470 480 EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR 490 500 510 WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 58.2; Estimated by SDS-PAGE under reducing condition (kDa): 85-90 (major) and 65-70 (minor)
Calculated PI :
6.92
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 92080
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
MICA; MIC-A; PERB11.1
Research Area :
Immunology
Pathway/Disease :
NK Cell Lectin-like Receptor
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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