Name :
Human CD72/LYB2/CD72b Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
B-cell differentiation antigen CD72, also known as Lyb2, is a single pass type II transmembrane glycoprotein that plays an important role in adaptive immunity. CD72 contains a 95 amino acid (aa) cytoplasmic domain with 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain (ECD) with a coiled-coil domain and a C-type lectin domain. CD72 is expressed on B lineage cells including Pre-B-cells and B-cells but not terminally differentiated plasma cells, NK cells, monocytes, dendritic cells, and mast cells. CD5 exists as a disulfide-linked homodimer. It interacts with CD5 and with CD100/Semaphorin 4D. CD5 also associates with CD79a in the B cell antigen receptor (BCR) complex following antigen stimulation. CD5 inhibits BCR signaling through interactions with the phosphatase PTPN6/SHP-1. CD72 ligation with antibodies or with CD100 induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP-1, leading to B cell proliferation. CD100 is expressed on activated NK cells and the adhesive interaction between CD100+ NK cells and CD72+ cells leads to an enhanced killing and IFNγ secretion. Both CD72 and CD100 are required for the maintenance of B cell anergy and tolerance. Mice that lack either CD72 or CD100 develop autoimmunity. CD72 may also induce positive signaling in B cells independent of the BCR signaling.
Gene Symbol :
NCBI Gene ID :
971
Uniprot Entry :
P21854
Construct Details :
The recombinant human CD72-Fc protein is expressed as a 468-amino acid protein consisting of Gln120 – Glu359 region of CD72 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD72-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QVSQQLQQTN RVLEVTNSSL RQQLRLKITQ LGQSAEDLQG SRRELAQSQE ALQVEQRAHQ 70 80 90 100 110 120 AAEGQLQACQ ADRQKTKETL QSEEQQRRAL EQKLSNMENR LKPFFTCGSA DTCCPSGWIM 130 140 150 160 170 180 HQKSCFYISL TSKNWQESQK QCETLSSKLA TFSEIYPQSH SYYFLNSLLP NGGSGNSYWT 190 200 210 220 230 240 GLSSNKDWKL TDDTQRTRTY AQSSKCNKVH KTWSWWTLES ESCRSSLPYI CEMTAFRFPD 250 260 270 280 290 300 STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV 310 320 330 340 350 360 DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA 370 380 390 400 410 420 KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD 430 440 450 460 SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 53.2 Estimated by SDS-PAGE under reducing condition (kDa): 55-60.
Calculated PI :
8.26
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 85215
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to human CD5 (SKU#FCL2664) and CD100 proteins, and induces IFNγ secretion in human natural killer NK92 lymphoma cells
Molecule Class :
1-Pass Type II Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD72; LYB2; CD72b; Lyb-2
Research Area :
Immunology
Pathway/Disease :
BCR Signaling
Species :
Human
CD Antigen :
CD72
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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