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Name :
Human CD5/Leu1/Ly1/T1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant

Description :
T-cell surface glycoprotein CD5, also known as Leu­1, Ly­1, and T1, is a single pass type I transmembrane protein belonging to the scavenger receptor superfamily. CD5 contains a 348 amino acid (aa) extracellular domain (ECD) with 3 scavenger receptor cysteine­rich (SRCR) domains. Crystal structure of the 3rd ECD of CD5 reveals the fold of a group B SRCR domain. The ECD of CD5 can be cleaved and circulates in the serum. T cell antigen receptor (TCR) positively regulates CD5 expression on developing thymocytes and on peripheral CD4+ T cells. CD5 is known to inhibit TCR signaling and promotes T cell anergy and survival. CD5 signaling negatively regulates the generation of regulatory T cells (Treg) but promotes the development of Th17 cells. CD5 interacts with CD72/Lyb-2 on B cells and with PTPN6/SHP-1 for intracellular signaling. Within the B cell lineage, CD5 is expressed on B­1a cells, anergic B cells, and IL­10 producing regulatory B cells. Similar to its role on T cells, CD5 negatively regulates signaling through the B cell antigen receptor (BCR) and supports B cell survival, anergy, and tolerance. CD5 is also involved in the cellular entry of hepatitis C virus into T lymphocytes.

Gene Symbol :

NCBI Gene ID :
921

Uniprot Entry :
P06127

Construct Details :
The recombinant human CD5-Fc protein is expressed as a 576-amino acid protein consisting of Arg25 – Pro372 region of CD5 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.

Source :
Human cells stably expressing human CD5-Fc and growing in chemical-defined media with no animal components or antibiotics

Amino Acid Sequence: :
10 20 30 40 50 60 RLSWYDPDFQ ARLTRSNSKC QGQLEVYLKD GWHMVCSQSW GRSSKQWEDP SQASKVCQRL 70 80 90 100 110 120 NCGVPLSLGP FLVTYTPQSS IICYGQLGSF SNCSHSRNDM CHSLGLTCLE PQKTTPPTTR 130 140 150 160 170 180 PPPTTTPEPT APPRLQLVAQ SGGQHCAGVV EFYSGSLGGT ISYEAQDKTQ DLENFLCNNL 190 200 210 220 230 240 QCGSFLKHLP ETEAGRAQDP GEPREHQPLP IQWKIQNSSC TSLEHCFRKI KPQKSGRVLA 250 260 270 280 290 300 LLCSGFQPKV QSRLVGGSSI CEGTVEVRQG AQWAALCDSS SARSSLRWEE VCREQQCGSV 310 320 330 340 350 360 NSYRVLDAGD PTSRGLFCPH QKLSQCHELW ERNSYCKKVF VTCQDPNPST GTHTCPPCPA 370 380 390 400 410 420 PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP 430 440 450 460 470 480 REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL 490 500 510 520 530 540 PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT 550 560 570 VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK

M.W. :
Calculated molecular mass (kDa): 64.2 Estimated by SDS-PAGE under reducing condition (kDa): 70-75.

Calculated PI :
8.05

Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 93080

Endotoxin Level :
>92% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)

Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).

Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Biological Activity :
Binds to human CD72 (SKU#FCL2666) measured by its binding ability in a functional ELISA.

Molecule Class :
1-Pass Type I Transmembrane

Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

Gene Family :
CD5; Leu-1; Ly-1; T1; LEU1; Ly1

Research Area :
Immunology

Pathway/Disease :
TCR Signaling

Species :
Human

CD Antigen :
CD5

References :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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