Name :
Human CD47/IAP/OA3/MER6 protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD47, also known as IAP and OA3, is a variably glycosylated transmembrane protein of the immunoglobulin superfamily (IgSF). It contains an extracellular domain (ECD) with a single Ig-like domain, five transmembrane domains with short intervening loops and a 34 amino acid cytoplasmic tail at the C-terminus. CD47 is broadly expressed. It is involved in the increase in intracellular calcium concentration in response to cell adhesion to extracellular matrix. By acting as an adhesion receptor, CD47 plays a role in both cell adhesion by positively or negatively modulating Integrin-mediated function. CD47 also binds to the SIRP family members on macrophages, neutrophils, and T lymphocytes, preventing macrophagemediated clearance of CD47expressing cells and promoting immune cell transmigration across the vascular endothelium. CD47 binds SIRPA which prevents maturation of dendritic cells and inhibits cytokine production. CD47 also interacts with SIRPG that enhances superantigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. CD47 is also a receptor for thrombospondin, and inhibits the angiogenic and vasorelaxant effects in the vasculature. On dendritic cells and myeloma cells, CD47 binds TSP-1 and induces giant cell formation and osteoclast differentiation.
Gene Symbol :
NCBI Gene ID :
961
Uniprot Entry :
Q08722
Construct Details :
The recombinant human CD47-Fc fusion is expressed as a 361 amino acid protein consisting of Gln19 – Asp141 region of CD47 (Uniprot Accession #Q08722) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD47-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP 70 80 90 100 110 120 TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS 130 140 150 160 170 180 PNESTTENLY FQGSTGTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV 190 200 210 220 230 240 SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK 250 260 270 280 290 300 ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ 310 320 330 340 350 360 PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K M.W.: Calculated molecular mass (kDa): 41; Estimated by SDS-PAGE under reducing condition (kDa): 60-65 Calculated PI: 6.12 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 54360 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Binds human SIRPA and SIRPG proteins. Blocks red blood cell adhesion to immobilized SIRPA protein. Inhibits CD47-mediated signaling activity. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Cancer Res. 52:5416 (1992). 2. Science 288:2051 (2000). 3. Trends Cell Biol. 11: 130 (2001). 4. J. Immunol. 168:5832 (2002). 5. Blood 109:836 (2007). 6. Curr. Drug Targ. 9:842 (2009). Documentation Product datasheet (pdf) can be downloaded here: FCL2233-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 6.12
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds human SIRPA and SIRPG proteins. Blocks red blood cell adhesion to immobilized SIRPA protein. Inhibits CD47-mediated signaling activity.
Molecule Class :
5-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD47; IAP; OA3; MER6
Research Area :
Immunology
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD47
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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