Name :
Human TAJ Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
TAJ (Toxicity and JNK inducer), also known as TRADE and TROY, is a single-pass type I membrane glycoprotein in the tumor necrosis factor receptor superfamily (TNFRSF19). TAJ contains 3 TNFR cysteine-rich repeats in the extracellular region but lacks a cytoplasmic death domain (DD). TAJ is widely expressed in the embryo, but shows restricted expression in adult. TAJ is detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles. TAJ has been shown to interact with TRAF family members to activate JNK signaling pathway when overexpressed in cells. TAJ is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TAJ may be a component of the NogoA receptor that mediates axon repulsion initiated by one of five myelinassociated inhibitory factors. In the hair follicle, TROY binds Lymphotoxin-α and acts with EDA to drive secondary hair follicle development. TAJ has also been found on melanoma cells, not normal melanocytes. TAJ has thus been identified as a biomarker for melanoma and represents a promising cell surface target for cell and antibody-based immunotherapies.
Gene Symbol :
TNFRSF19; TROY; TRADE; TAJ-alpha; UNQ1888/PRO4333
NCBI Gene ID :
55504
Uniprot Entry :
Q9NS68
Construct Details :
The recombinant human TAJ-Fc fusion is expressed as a 368 amino acid protein consisting of Glu30 – Ala169 region of TAJ (UniProt accession #Q9NS68) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human TAJ-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 ESGDCRQQEF RDRSGNCVPC NQCGPGMELS KECGFGYGED AQCVTCRLHR FKEDWGFQKC 70 80 90 100 110 120 KPCLDCAVVN RFQKANCSAT SDAICGDCLP GFYRKTKLVG FQDMECVPCG DPPPPYEPHC 130 140 150 160 170 180 ASKVNLVKIA STASSPRDTA STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV 190 200 210 220 230 240 TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY 250 260 270 280 290 300 KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV 310 320 330 340 350 360 EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass (kDa): 40.9; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
6.82
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 46755
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Not Available
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TNFRSF19; TROY; TRADE; TAJ-alpha; UNQ1888/PRO4333
Research Area :
Development
Pathway/Disease :
Hair Follicle Development
Species :
Human
CD Antigen :
References :
1. Genomics 62:103 (1999). 2. J. Biol. Chem. 275:15336 (2000). 3. J. Biol. Chem. 275:20742 (2000). 4. Neuron 45:345 (2005). 5. Hum. Mol. Genet. 17:3380 (2008).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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