Name :
Human KIT/SCFR/PBT/CD117/C-KIT Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
KIT, also known as c-KIT, SCFR (stem cell factor receptor), PBT (Piebald trait protein), and CD117, is a single pass type I transmembrane protein belonging to the receptor tyrosine kinase (RTK) family and the CSF-1/PDGF receptor subfamily. KIT is a cellular homolog of the feline sarcoma virus protein, v-Kit. Kit contains 5 immunoglobulin (Ig)-like C2-type domains in the extracellular domain (ECD) and 1 split tyrosine kinase domain in the cytoplasmic region. Alternative splicing of KIT generates a potentially secreted isoform corresponding to the first 4 Ig-like domains. KIT may act as cell-surface receptor for the cytokine KITLG/SCF and play an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF, KIT can activate PI3K and AKT1 signaling pathways. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases, MAPK1/ERK2 and/or MAPK3/ERK1. KIT promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Mutations in the KIT gene are associated with gastrointestinal stromal tumors (GIST), mast cell disease, acute myelogenous leukemia (AML), and piebaldism. KIT protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction.
Gene Symbol :
KIT; PBT; SCFR; C-Kit; CD117
NCBI Gene ID :
3815
Uniprot Entry :
P10721
Construct Details :
The recombinant human KIT-Fc protein is expressed as a 737-amino acid protein consisting of Gln26 – Pro524 region of KIT with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human KIT-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QPSVSPGEPS PPSIHPGKSD LIVRVGDEIR LLCTDPGFVK WTFEILDETN ENKQNEWITE 70 80 90 100 110 120 KAEATNTGKY TCTNKHGLSN SIYVFVRDPA KLFLVDRSLY GKEDNDTLVR CPLTDPEVTN 130 140 150 160 170 180 YSLKGCQGKP LPKDLRFIPD PKAGIMIKSV KRAYHRLCLH CSVDQEGKSV LSEKFILKVR 190 200 210 220 230 240 PAFKAVPVVS VSKASYLLRE GEEFTVTCTI KDVSSSVYST WKRENSQTKL QEKYNSWHHG 250 260 270 280 290 300 DFNYERQATL TISSARVNDS GVFMCYANNT FGSANVTTTL EVVDKGFINI FPMINTTVFV 310 320 330 340 350 360 NDGENVDLIV EYEAFPKPEH QQWIYMNRTF TDKWEDYPKS ENESNIRYVS ELHLTRLKGT 370 380 390 400 410 420 EGGTYTFLVS NSDVNAAIAF NVYVNTKPEI LTYDRLVNGM LQCVAAGFPE PTIDWYFCPG 430 440 450 460 470 480 TEQRCSASVL PVDVQTLNSS GPPFGKLVVQ SSIDSSAFKH NGTVECKAYN DVGKTSAYFN 490 500 510 520 530 540 FAFKGNNKEQ IHPHTLFTPS TTENLYFQGS TGTHTCPPCP APELLGGPSV FLFPPKPKDT 550 560 570 580 590 600 LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH 610 620 630 640 650 660 QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK NQVSLTCLVK 670 680 690 700 710 720 GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE 730 ALHNHYTQKS LSLSPGK
M.W. :
Calculated molecular mass (kDa): 82.8 Estimated by SDS-PAGE under reducing condition (kDa): 90-100.
Calculated PI :
6.27
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 106325
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds its ligand KITL/SCF (SKU#FCL2346 and #FCL2384) and neutralizes its mediated signaling activity.
Molecule Class :
Receptor Tyrosine Kinase (RTK)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
KIT; PBT; SCFR; C-Kit; CD117
Research Area :
Cancer
Pathway/Disease :
CSF Signaling Pathway
Species :
Human
CD Antigen :
CD117
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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