Name :
Human CSF1R/FMS/MCSFR/CD115 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CSF1R (colony stimulating factor 1 receptor), also known as M-CSFR (macrophage colony-stimulating factor receptor) and CD115, is encoded by the proto-oncogene c-fms. CSF1R is a 1 pass type I transmembrane protein of the CSF1/PDGF receptor subfamily of receptor tyrosine kinases (RTKs). Like other members, CSF1R contains 5 immunoglobulinlike (Ig) domains in their extracellular region and a tyrosine kinase domain in their intracellular region. CSF1R acts as a receptor for CSF1 and IL34, and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. CSF1R promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. CSF1R promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. CSF1R also regulates osteoclast proliferation and differentiation and is required for normal bone and tooth development. CSF1R is also required for normal male and female fertility, and for development of milk ducts and acinar structures in the mammary gland during pregnancy. CSF1R activates several signaling pathways including PKC, AKT MAPK and SRC kinase pathways. CSF1R also activates STAT family members. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy. Aberrant expression of CSF1R is observed in breast, ovarian, prostate, and endometrial cancers as well as inflammatory diseases.
Gene Symbol :
CSF1R; FMS; M-CSFR; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
NCBI Gene ID :
1436
Uniprot Entry :
P07333
Construct Details :
The recombinant human CSF1R-Fc is expressed as a 736 -amino acid protein consisting of Ile20 – Pro517 region of CSF1R with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CSF1R-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 IPVIEPSVPE LVVKPGATVT LRCVGNGSVE WDGPPSPHWT LYSDGSSSIL STNNATFQNT 70 80 90 100 110 120 GTYRCTEPGD PLGGSAAIHL YVKDPARPWN VLAQEVVVFE DQDALLPCLL TDPVLEAGVS 130 140 150 160 170 180 LVRVRGRPLM RHTNYSFSPW HGFTIHRAKF IQSQDYQCSA LMGGRKVMSI SIRLKVQKVI 190 200 210 220 230 240 PGPPALTLVP AELVRIRGEA AQIVCSASSV DVNFDVFLQH NNTKLAIHQQ SDFHNNRYQK 250 260 270 280 290 300 VLTLNLDQVD FQHAGNYSCV ASNVQGKHST SMFFRVVESA YLNLSSEQNL IQEVTVGEGL 310 320 330 340 350 360 NLKVMVEAYP GLQGFNWTYL GPFSDHQPEP KLANVTTKDT YRHTFTLSLP RLKPSEAGRY 370 380 390 400 410 420 SFLARNPGGW RALTFELTLR YPPEVSVIWT FINGSGTLLC AASGYPQPNV TWLQCSGHTD 430 440 450 460 470 480 RCDEAQVLQV WDDPYPEVLS QEPFHKVTVQ SLLTVETLEH NQTYECRAHN SVGSGSWAFI 490 500 510 520 530 540 PISAGAHTHP PDEFLFTPST TENLYFQGST GTHTCPPCPA PELLGGPSVF LFPPKPKDTL 550 560 570 580 590 600 MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ 610 620 630 640 650 660 DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG 670 680 690 700 710 720 FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA 730 LHNHYTQKSL SLSPGK
M.W. :
Calculated molecular mass (kDa): 81.9 Estimated by SDS-PAGE under reducing condition (kDa): 90-100.
Calculated PI :
6.22
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 116740
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds human CSF1 (SKU#FCL2669) and neutralizes CSF1-mediated signaling activity including CSF1-induced proliferation of leukemia lymphoblast cells.
Molecule Class :
Receptor Tyrosine Kinase (RTK)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CSF1R; FMS; M-CSFR; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Research Area :
Cancer
Pathway/Disease :
CSF & IL34 Signaling Pathway
Species :
Human
CD Antigen :
CD115
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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