Name :
Human DR5 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
DR5 (death receptor 5), also known as CD262, TRAILR2, KILLER, and TRICK2, is a single pass type 1 transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF10B). DR5 contains an extracellular region with 3 TNFR cysteine-rich repeats and a cytoplasmic death domain (DD). DR5 is widely expressed in normal tissues with high levels in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract. DR5 is very highly expressed in various tumor cell lines. DR5 is one of 5 receptors for TRAIL (tumor necrosis factor-related apoptosis inducing ligand), also known as Apo2L (Apo-2 ligand) and TNFSF10. TRAIL preferentially induces apoptosis in tumor cell lines, but not in normal cells. TRAIL binds at least 5 different receptors with characteristic TNFR cysteine-rich repeats. DR5/TRAILR2 is most closely related to DR4/TRAILR1 sharing 59% amino acid identity within the cytoplasmic DD. DR5 transduces cell death signal and induces apoptosis by recruiting a cytosolic deathinducing signaling complex (DISC) that includes the adaptor FADD and caspase 8 and caspase 10. DR5 also promotes the activation of NF-κB and plays an essential role in ER stress-induced apoptosis. Defects in DR5 may be a cause of head and neck squamous cell carcinomas (HNSCC).
Gene Symbol :
DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5; TNFRSF10B
NCBI Gene ID :
8795
Uniprot Entry :
O14763
Construct Details :
The recombinant human DR5-Fc fusion is expressed as a 381 amino acid protein consisting of Ile56 – Pro208 region of DR5 (UniProt accession #O14763) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human DR5-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 ITQQDLAPQQ RAAPQQKRSS PSEGLCPPGH HISEDGRDCI SCKYGQDYST HWNDLLFCLR 70 80 90 100 110 120 CTRCDSGEVE LSPCTTTRNT VCQCEEGTFR EEDSPEMCRK CRTGCPRGMV KVGDCTPWSD 130 140 150 160 170 180 IECVHKESGT KHSGEVPAVE ETVTSSPGTP ASPSTGTHTC PPCPAPELLG GPSVFLFPPK 190 200 210 220 230 240 PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL 250 260 270 280 290 300 TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT 310 320 330 340 350 360 CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS 370 380 VMHEALHNHY TQKSLSLSPG K
M.W. :
Calculated molecular mass (kDa): 42.3; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
5.98
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 50640
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand human TRAIL and inhibits TRAIL-mediated signaling activity including TRAIL-induced cytotoxicity in L-929 mouse fibroblast cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5; TNFRSF10B
Research Area :
Immunology
Pathway/Disease :
Apoptosis
Species :
Human
CD Antigen :
CD262
References :
1. J. Biol. Chem. 272:25417 (1997). 2. Science 277:815-818(1997). 3. Immunity 7:821 (1997). 4. EMBO J. 16:5386 (1997). 5. J Immunol. 171: 1061 (2003). 6. J. Biol. Chem. 279:45495 (2004).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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