Name :
Human AXL/UFO Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
Axl, also known as Ufo and Ark, is a single-pass type I transmembrane glycoprotein in the TAM receptor tyrosine kinase (RTK) family. Like other family members Tyro3 and Mer, Axl contains two immunoglobulinlike (Ig) domains and two fibronectin type III domains in the extracellular region and a cytoplasmic tyrosine kinase domain. The TAM receptors are involved in regulation of immune response, cell survival and migration as well as tumorigenesis and metastasis. Axl binds to its ligand Gas6 (growth arrest specific protein 6), which trIgGers tyrosine auto-phosphorylation and activation of Axl. Axl induces a wide range of activities including platelet aggregation and thrombus formation, macrophage and dendritic cell phagocytosis, NK cell development, and angiogenesis. Axl is upregulated in various disease states, such as in multiple sclerosis (MS) lesions. Axl contributes to vascular remodeling and inflammatory cell infiltration. Axl acts as a cellular entry receptor for Gas6opsonized lentiviruses. Axl also contributes to tumor cell survival, migration, invasion, metastasis and chemosensitivity. Axl is highly expressed in solid cancers and mediates multiple oncogenic phenotypes. Axl expression correlates with poor prognosis in several cancers. A soluble form of the Axl ectodomain, which is shed by proteolytic cleavage, retains the ability to bind Gas6 and blocks its signaling, showing the therapeutic potential in the inhibition of metastatic tumor progression.
Gene Symbol :
AXL; UFO; JTK11
NCBI Gene ID :
558
Uniprot Entry :
P30530
Construct Details :
The recombinant human AXL-Fc fusion is expressed as a 650 amino acid protein consisting of Ala26 – Ser447 region of AXL and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing AXL-Fc and growing in chemical-defined media with no animal components or antibiotics.
Amino Acid Sequence: :
APRGTQAEESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQ VPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEP EDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSC EAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLS DDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLP VETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGL RQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPA FSSTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNW YVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTIS KAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV LDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK M.W.: Calculated molecular mass (kDa): 71.1; Estimated by SDS-PAGE under reducing condition (kDa): 80-90 Calculated PI: 5.35 Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 97580 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free). Endotoxin Level: Biological Activity: Interacts with human Gas6 and inhibits Gas6/AXLmediated signaling activity.
M.W. :
Calculated molecular mass (kDa): 71.1; Estimated by SDS-PAGE under reducing condition (kDa): 80-90
Calculated PI :
5.35
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Interacts with human Gas6 and inhibits Gas6/AXLmediated signaling activity.
Molecule Class :
Receptor Tyrosine Kinase (RTK)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
UFO; JTK11
Research Area :
Cancer
Pathway/Disease :
Gas6 Signaling Pathway
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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