Name :
Human CD123/IL3RA/IL3R Protein, ECD (Extracellular Domain), Recombinant
Description :
CD123, also known as IL3RA/IL3R (Interleukin-3 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily. CD123/IL3RA consists of an extracellular region and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif. The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD123/IL3RA is a component of the receptor complex specific for IL3. The IL3 receptor complex is a heterodimer of CD123/IL3RA and the common signal transducing subunit CD131/CSF2RB shared by the receptors for IL3, colony stimulating factor 2 (CSF2/GM-CSF), and IL5. The binding of CD123/IL3RA to IL3 depends on the beta subunit CD131. The beta subunit CD131 is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA/CD116) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y.
Gene Symbol :
10 20 30 40 50 60
NCBI Gene ID :
3563
Uniprot Entry :
P26951
Construct Details :
The recombinant human CD123 ECD protein is expressed as a 306 amino acid protein consisting of Thr19 – Ala303 region of CD123 and a C-terminal His-tag. It contains 6 potential sites for N-linked glycosylation.
Source :
Human cells stably expressing CD123 ECD and growing in chemical-defined media with no animal component or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 TKEDPNPPIT NLRMKAKAQQ LTWDLNRNVT DIECVKDADY SMPAVNNSYC QFGAISLCEV 70 80 90 100 110 120 TNYTVRVANP PFSTWILFPE NSGKPWAGAE NLTCWIHDVD FLSCSWAVGP GAPADVQYDL 130 140 150 160 170 180 YLNVANRRQQ YECLHYKTDA QGTRIGCRFD DISRLSSGSQ SSHILVRGRS AAFGIPCTDK 190 200 210 220 230 240 FVVFSQIEIL TPPNMTAKCN KTHSFMHWKM RSHFNRKFRY ELQIQKRMQP VITEQVRDRT 250 260 270 280 290 300 SFQLLNPGTY TVQIRARERV YEFLSAWSTP QRFECDQEEG ANTRASTTEN LYFQGSTGHH HHHHHH
M.W. :
Calculated molecular mass (kDa): 35.2; Estimated by SDS-PAGE under reducing condition (kDa): 60-65
Calculated PI :
8.30
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 55515
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free)
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Research Area :
Immunology
Pathway/Disease :
Cytokine Signaling
Species :
Human
CD Antigen :
CD123
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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