D between conditions. We aimed to detect non-linear effects of remedy with silimarin. We have detected octamer RNA sequences that are over-represented in the exosomes from stimulated cells and we’ve used these motifs to detect mRNAs associated to lipid metabolism that are additional likely to become the targets of micro RNAs with these particular motifs. We validated our predictions applying the available databases for miRNA-target interaction prediction (e.g. PITA, TargetScanS). We have used Renyi Divergence of order 0.5 to quantify the similarity of expression patterns of all transcripts, to a pre-selected set of miRNAs identified to become involved in inflammatory pathway, across all experimental conditions. We have performed hierarchical clustering to detect co-regulated micro RNAs. Outcomes: Amongst most substantially changed by inflammatory stimuli exosomal miRNAs were: hsa-let-7b-5p, targeting IRS2, involved in steatohepatitis, hsa-miR-6790-5p and hsa-miR-146b-5p, whereas silimarin affected exosomal presence of hsa-miR-146b-5p, hsa-miR-542-3p. mir146b was reported to straight influence TRAF6 and IRAK1 mRNA and protein levels in macrophages Summary/Conclusion: We have identified set of miRNAs, which presence in Hep2G exosomes is altered by inflammatory stimuli and which could potentially impact expression of genes involved in lipid metabolism in KCs. The precise influence of Hep2G-derived miRNA on KCs need additional investigation.LBP.Platelet derived-microparticles as modulator of plasmacytoid dendritic cell inflammatory response Adam Ceroi1, Sameh Obeid2, Thomas Cherrier3, C ine Elie-Caille2, Wilfried Boireau2 and Philippe SaasRavicahndran’s Lab., University of Virgina, VA, USA; 2Institut FEMTO-ST, CNRS, Univ. Bourgogne Franche Comte; 3INSERM UMR 1098, ESFBourgogne-Franche Comt Univ. de Franche-ComteLBP.Exosomal miRNA in Hep2G cells stimulated by pro-inflammatory cytokines Justyna Toto-uraska1, Michal Seweryn1, Katarzyna Poskrubek2, Anna Winiewska2, Rafal Olszanecki2, Pawel Wolkow3 and Ryszard Korbut1 NLRP3 Storage & Stability Jagiellonian University Health-related College Center for Health-related Genomics OMICRON, Krakow, Poland; 2Jagiellonian University Health-related College Division of Pharmacology, Krakow, Poland; 3Jagiellonian University Health-related College Center for Health-related Genomics – OMICRON, Jagiellonian university Health-related College Department Of Pharmacology, Krakow, Poland;Introduction: Microparticles (MP) are generated in the plasma membrane of parental cells immediately after activation or cell death. Depending on the stimulus accountable for MP generation, it was demonstrated that the quantity, Aromatase review content material and biological activities of MP may well vary. Previously, we demonstrated that platelet or endothelial cell-derived MP uptake by plasmacytoid dendritic cells (PDC) can modulate the Liver X Receptor (LXR) pathway, then regulate inflammatory responses. Here, we employed MP from resting- or collagen activated-platelet (rest-PMP or colPMP, respectively) to compare their size and protein content material, and investigate their effect on the LXR pathway plus the subsequent inflammatory response in PDC. Methods: Platelet from wholesome donors have been stimulated or not by collagen, and platelet derived-MP (PMP) have been isolated by centrifugation. PMP size and concentration have been investigated by flow cytometry and Surface Plasmon Resonance coupled with Atomic Force Microscopy, followed by Mass Spectrometry to ask their protein content material. Utilizing PDC from wholesome donors, we investigated LXR pathway involvement (by means of LXR-target gene.
