S doable that NaPaC administrated early was in a position to inactivate, at the least in aspect, this development element and consequently to prevent vessel dilation. Due to the fact vessels are present even within the early treated tumours, it could be that A431 cells surround and co-opt, straight away immediately after inoculation, the existing subcutaneous vessels since it was described inside the case of non-small-cell-lung carcinoma (Pezzela et al, 1997) and melanoma (Leenders et al, 2002). Moreover, NaPaC seems to possess no impact, administrated early or late, on this phenomenon. On the other hand, we can’t discard that in our experimental model the IL-17 Antagonist Accession formation of neo-vessels happens really early and that NaPaC is just not capable to inhibit it entirely. Altogether, our benefits showed that NaPaC inhibited the A431 tumour development acting on each endothelial and tumour cells. The extent of this effect was dependent around the outset of NaPaC remedy. Because the period of NaPaC action on A431 cell proliferation was the same (5 weeks) and because the endothelial cell density was decreased inside the identical manner in each early and late treated tumours, essentially the most probable is the fact that the distinction in tumour growth inhibition was due to modifications in intratumour vascular network top for the enhance in tumour cell death observed above. Altogether, our data indicate that A431 xenograft model might be made use of to study the influence of vascular network in tumour development and to screen possible antiangiogenic agents. In conclusion, we demonstrated that NaPaC potently inhibits fast-growing epidermoid carcinoma by acting on tumour cells and intratumour endothelial cells whatever the state of xenograft development. Nontoxic at efficient doses, NaPaC provides interesting clues for therapies of solid Bcl-2 Inhibitor review tumours stopping the vascular network evolution in malignant lesions, as a result inhibiting the speedy expansion from modest tumours to late-stage tumours. Additionally, its direct inhibitory action on tumour cell proliferation argues for its usefulness in late-stage tumour remedy.ACKNOWLEDGEMENTS` We thank Grant sponsors: Ministere de l’Education Nationale; Association pour la Recherche contre le Cancer (Grant no. 9721), La Ligue Nationale contre le Cancer and Biodex Laboratory. We are grateful to Professor A Martin for helpful discussions concerning the histological tumour analysis, Professor M Frojmovic for English corrections, O Sainte-Catherine for exceptional technical help and L Correa for NaPaC preparation. We thank Professor PM Martin for A431 cell present.
PO Box 2345, Beijing 100023, China Fax: +86-10-85381893 E-mail: [email protected] www.wjgnet.comWorld J Gastroenterol 2004;10(23):3414-3418 World Journal of Gastroenterology Copyright 2004 by The WJG Press ISSN 1007-LIVER CANCER Expressions of cysteine-rich61, connective tissue growth aspect and Nov genes in hepatocellular carcinoma and their clinical significanceZhi-Jun Zeng, Lian-Yue Yang, Xiang Ding, Wei WangZhi-Jun Zeng, Lian-Yue Yang, Xiang Ding, Wei Wang, Liver Cancer Laboratory, Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China Supported by the National Key Technologies R and D Plan, No. 2001BA703BO4 as well as the National Organic Science Foundation of China, No.30371595 Correspondence to: Lian-Yue Yang, Liver Cancer Laboratory, Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. [email protected] Phone: +86-731-4327326 Fax: +86-731-4327332 Received: 2004-02-28 Accepted:.
