Values, we detected negative or negligible not significant values for each
Values, we detected unfavorable or negligible not considerable values for each ABTS (-0.155) and FRAP (0.098). Though pumpkins are viewed as a great source of carotenoids, the results obtained within this study apparently seem to indicate that carotenoids do not play a significant function within the antioxidant capacity within the accessions chosen. It’s well-known that carotenoids are generally incredibly unstable molecules. As a result, storage, sample preparation, along with other unidentified things could account for these not substantial correlation values [23,45]. Even so, it can be possible that the distinct phytocompounds as discerned by the discriminant terpenoids identified by the VIP strategy reported in Tables 1 and 2, on both the origin with the pumpkin accessions and on the two pumpkin species, respectively, could contribute to the in vitro antioxidant capacity values reported. Notwithstanding the outcomes obtained, carotenoids are regarded as potent antioxidants and as such can contribute towards the prevention of aging and also the development of some ailments including atherosclerosis along with other cardiovascular illnesses [42,44,46]. They will also guard the eye and macula from degradation, with zeaxanthin being the predominant carotenoid with the macula pigment and therefore vital for eye overall health [47]. In our study, the results of carotenoid quantification on the pumpkin accessions show that they decreased inside the following order of concentration: -carotene -carotene zeaxanthin lutein. These four carotenoids have been chosen considering that they’re the most widespread among the Cucurbitaceae loved ones [48]. The total carotenoids found in the present study are comparable to those reported inside the literature for pumpkin solutions from C. maxima and C. moschata, which include in pumpkin pulp flour [23,49]. Interestingly, on comparing the pumpkin samples determined by their origin, we noticed that, normally, those of Serbian origin had been characterized by the presence of each -carotene and -carotene, whilst in these of non-Serbian origin, the presence of -carotene and zeaxanthin was dominant. The outcomes demonstrate that there is certainly Aztreonam Protocol higher diversity in carotenoid form and concentration inside the various Cucurbitaceae Polmacoxib supplier species and cultivars, and this can be in accordance with numerous literature reports. For example, Kulczynski and Gramza-Michalowska [27] located that lutein was by far the most abundant carotenoid among 11 C. maxima cultivars, contrary to our benefits, exactly where it was detected in the lowest amount, whereas -carotene was one of the most abundant amongst the C. maxima cultivars in our study. Inside a prior study by Kulaitiene et al. [50] on cultivars of the C. maxima species, lutein and zeaxanthin were essentially the most abundant carotenoids. Azevedo-Meleiro and Rodriguez-Amaya [10] also located various carotenoids, based on the pumpkin species and cultivar, with C. moschata cultivars possessing higher contents of -carotene too as -carotene and smaller sized amounts of lutein and neoxanthin. On cultivars of C. moschata species, each Norshazila et al. [51] and de Carvalho et al. [52] observed that -carotene was the predominant carotenoid. Nevertheless, a study by Murkovic et al. [28]Antioxidants 2021, ten,13 ofshowed that -carotene was probably the most dominant carotenoid in most tested pumpkin varieties in each C. maxima and C. moschata fruits from Austria. The above discrepancies might depend on the environmental and growing circumstances (climate, soil, sowing, harvesting, storage). On the other hand, when such factors are limited as in the present study, the g.