Product Name :
Anti-CD16a, Human antibody
Applications:
ELISA,Flow Cyt
Reactivity :
Human/Cynomolgus
Conjugate:
Unconjugated
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-CD16a, Human antibody is designed for detecting human CD16a specifically. Anti-CD16a, Human antibody is recombinant VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc. Based on ELISA, Anti-CD16a, Human antibody reacts with human CD16a, and has no reactivity with CD16b. | Immunogen: Recombinant human CD16a | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation) | Conjugate: Unconjugated | Specificity: Human CD16a | Cross-Reactivity: Cross-reactivity with cynomolgus CD16a | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C | Background:CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms – a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-CD16a, Human antibody is designed for detecting human CD16a specifically.{{909395-70-6} web|{909395-70-6} Protocol|{909395-70-6} Data Sheet|{909395-70-6} custom synthesis} Anti-CD16a, Human antibody is recombinant VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc. Based on ELISA, Anti-CD16a, Human antibody reacts with human CD16a, and has no reactivity with CD16b.{{571203-78-6} web|{571203-78-6} Technical Information|{571203-78-6} In stock|{571203-78-6} custom synthesis}
Immunogen:
Recombinant human CD16a
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to Human IgG1 Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Function:
ELISA: 1:4,000-1:10000Flow Cytometry：1:200-1:1000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc.PMID:29630258 The actual dilution used must be determined empirically.
