Product Name :
Anti-Human IgA, AlpSdAbs® VHH(Biotin)
Applications:
ELISA,IP,Purification,BLI,SPR
Reactivity :
Human IgA
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Human IgA, AlpSdAbs® VHH(Biotin) is designed for detecting human IgA specifically. Anti-Human IgA, AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibody to human IgA coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgA, AlpSdAbs® VHH(Biotin) reacts with human IgA chain selectively, no reactivity with human IgG, IgE, IgM, IgD.{{1809249-37-3} MedChemExpress|{1809249-37-3} Biological Activity|{1809249-37-3} Formula|{1809249-37-3} manufacturer} | Immunogen: Human IgA | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: Biotin-SP (long spacer) | Specificity: Human IgA | Cross-Reactivity: Does not bind to human IgG, IgE, IgM, IgD | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS(pH 7.{{887375-67-9} site|{887375-67-9} Biological Activity|{887375-67-9} Description|{887375-67-9} manufacturer} 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C | Background:In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgA is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(
Description2 :
Anti-Human IgA, AlpSdAbs® VHH(Biotin) is designed for detecting human IgA specifically. Anti-Human IgA, AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibody to human IgA coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgA, AlpSdAbs® VHH(Biotin) reacts with human IgA chain selectively, no reactivity with human IgG, IgE, IgM, IgD.
Immunogen:
Human IgA
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Function:
ELISA: 1:5000-1:20000IP: 1-2ug/sampleBLI (biolayer interferometry)SPR (surface plasmon resonance)Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc.PMID:30942050 The actual dilution used must be determined empirically.