Product Name :
Anti-Halo tag, AlpSdAbs® VHH(Biotin)
Applications:
ELISA,Purification,BLI,SPR
Reactivity :
Halo tag
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Halo tag, AlpSdAbs® VHH(Biotin) is designed for detecting Halo tag fusion proteins. Anti-Halo tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to Halo tag coupled to Biotin, and Anti-Halo tag, AlpSdAbs® VHH(Biotin) detects the Halo tag selectively, no reactivity with other proteins. | | Immunogen: Halo tag fused KLH | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: Biotin | Specificity: Halo tag | Cross-Reactivity: Highly selective for Halo tag sequence | Affinity: Dissociation constant KD of 0.{{112-80-1} MedChemExpress|{112-80-1} Technical Information|{112-80-1} In stock|{112-80-1} manufacturer} 1nM | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles) | Background:The protein tag(Halo tag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (Halo tag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Due to the single-chain nature of sdAbs and their increased sensitivity and higher affinity to Halo tag fusion proteins, Anti-Halo Tag, Rabbit antibody can be used to detect the Halo tag selectively, with high sensitivity and negligible non-specific background.
Description2 :
Anti-Halo tag, AlpSdAbs® VHH(Biotin) is designed for detecting Halo tag fusion proteins. Anti-Halo tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to Halo tag coupled to Biotin, and Anti-Halo tag, AlpSdAbs® VHH(Biotin) detects the Halo tag selectively, no reactivity with other proteins.{{2519537-70-1} web|{2519537-70-1} Technical Information|{2519537-70-1} In Vivo|{2519537-70-1} supplier}
Immunogen:
Halo tag fused KLH
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
10mM PBS (pH 7.PMID:29939596 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage :
Store at –20 °C(Avoid freeze / thaw cycles)
Function:
WB: 1:5,000-1:20000 ELISA: 1:5,000-1:20000IP: 1:100-1:500Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
