Hvir: Heliothis virescens, Hzea: Helicoverpa zea, Lcup: Lucilia cuprina, Ldec: Leptinotarsa decemlineata, Mdom: Musca domestica, Mper: Myzus persicae, Ncin: Nephotettix cincticeps, Nlug: Nilaparvata lugens, Ppol: Papilio polyxenes, Sgra: Schizaphis graminum, Tcas: Tribolium castaneum, Tmol: Tenebrio molitor. (ZIP)Acknowledgments“We thank Aris Ilias (University of Crete) for his assist with the cloning and sequencing of P450s.length and sequence, clade and clade identification approach of contigs encoding for putative B. oleae P450s. (XLSX) Sequence name, accession quantity, nucleotide and amino acid length and sequence, clade and clade identification process of B. oleae P450s obtained by degenerate PCR. (XLSX)Table SAuthor ContributionsConceived and created the experiments: NP JV. Performed the experiments: NP WD SR AC. Analyzed the information: NP WD SR Television. Contributed reagents/materials/analysis tools: AC SR. Wrote the paper: NP WP JV. Created the software used in analysis: SR.
Phillips et al. BMC Pregnancy and Childbirth 2014, 14:241 http://www.biomedcentral/1471-2393/14/RESEARCH ARTICLEOpen AccessProstaglandin pathway gene expression in human placenta, amnion and choriodecidua is differentially affected by preterm and term labour and by uterine inflammationRobert J Phillips1, Michel A Fortier2 and Andr L ez Bernal1,3*AbstractBackground: Elucidation of the biochemical pathways involved in activation of preterm and term human labour would facilitate the development of helpful management and inform judgements with regards to the necessity for preterm tocolysis and post-term induction. Prostaglandins act at all stages of human reproduction, and are potentially activators of labour. Solutions: Expression of 15 genes involved in prostaglandin synthesis, transport and degradation was measured by qPCR using tissue samples from human placenta, amnion and choriodecidua at preterm and full-term vaginal and caesarean delivery.Pelabresib Cellular localisation of eight prostaglandin pathway proteins was determined by immunohistochemistry. Benefits: Expression of prostaglandin pathway genes was differentially affected by elements like gestational age at delivery, plus the incidence and duration of labour.Vancomycin Chorioamnionitis/deciduitis was related with upregulation of PTGS2 (prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)), in conjunction with the inflammatory genes IL8 (interleukin 8), S100A8 (S100 calcium binding protein A8) and TLR2 (toll-like receptor 2), in amnion and choriodecidua, and with downregulation of CBR1 (carbonyl reductase 1) and HPGD (hydroxyprostaglandin dehydrogenase 15-(NAD)) in choriodecidua.PMID:29844565 Protein localisation differed drastically in between the various maternal and fetal cell forms. Conclusions: Preterm and term labour are associated with distinct prostaglandin pathway expression profiles; inflammation provokes specific modifications, unrelated for the presence of labour; spontaneous and induced term labour are indistinguishable. Keyword phrases: Parturition, Inflammation, Pregnancy, UterusBackground Human labour requires a dramatic transition from a state of uterine quiescence and immune tolerance in the fetus–that prevails throughout pregnancy–to a short period of intense uterine activation involving connective tissue remodelling and coordinated smooth muscle activity. The signals that initiate this approach usually are not yet* Correspondence: [email protected] 1 Henry Wellcome Laboratories for Integrative Neuroscience and Endocrino.
