E the separated cardiomyocytes attain the desired survival price and purity may be the crucial troubles for cardiomyocyte culture in vitro. In operation, this paper makes the following improvements: Choose the mixed digestion solution with moderate function and applicable to cardiomyocyte digestion and separation, and it can speedily digest the tissue and will not injure cardiomyocytes. Through digestion, water bath temperature must be continual at 37 appropriately, the initial cardiomyocyte digestion time needs to be ten min, and0.6117 0.062* 0.8213 0.01*27.70.0.5442 0.025* 0.8021 0.038* 0.4804 0.13.28 66.Note: Evaluate to normal handle group, *P 0.Table 5: Result of effects of ferulic acid and its drug-containing plasma on the LDH activity within the myocardial cell culture supernatant and Na+-K+- ATPase and Ca2+- ATPase activity inside the myocardial cell (X s)Experimental group Blank control group Injured group High-concentration ferulic acid group Medium-concentration ferulic acid group Low-concentration ferulic acid group Drug-containing plasma containing higher concentrations of ferulic acid Drug-containing plasma containing medium Concentrations of ferulic acid Drug-containing plasma containing low concentrations of ferulic acidNote: Examine to regular control group, *P 0.LDH (U/L) 68.7 2.31* 218 7.13* 144 12.8* 172 five.21* 186 7.13* 128 3.92* 141 four.1* 113 8.38*Na+-K+- ATPase (umol.mg-.h-) 0.581 0.212* 0.478 0.077* 0.552 0.213* 0.514 0.032* 0.503 0.102* 0.562 0.411* 0.53 0.159* 0.571 0.613*Ca2+- ATPase (umol.mg-.h-) 0.791 0.125* 0.455 0.137* 0.613 0.121* 0.57 0.03* 0.541 0.031* 0.654 0.11* 0.611 0.024* 0.681 0.159*Pharmacognosy Magazine | July-September 2013 | Vol 9 | IssueRen, et al.: Protective effects of ferulic acid on principal cultured neonatal rat cardiomyocytesTable 6: Result of effects of ferulic acid and its drug-containing plasma on spontaneous frequency of myocardial cell (X s)Groups Before Following 1 2 45 14 0 min 73 14* 78 13* 5 min 75 12* 79 18* ten min 77 11* 81 15* Beating price 20 min 79 10* 82 16* 30 min 80 11* 83 10*Note: 1 = the optimal concentration of ferulic acid, two = the optimal concentration of ferulic acid containing plasma, Compare to standard handle group, *P 0.M-CSF Protein, Rat Table 7: Result of effects of ferulic acid and its drug-containing plasma on prospective across plasma membrane of myocardial cells (X s)Time/ min Prior to Right after 0 min 0 min five min five min ten min 10 min 20 min 20 min 30 min 30 min 1 2 1 two 1 two 1 2 1 two MDP (-mv) 68.AEE788 7 five.PMID:24179643 7* 65.four 1.91* 66.7 5.2* 65.6 two.1* 66.9 7.7* 65.9 2.3* 67.three 3.82* 67.two six.3* 69.0 five.8* 66.four 5.23* 68.three 4.8* APA (mv) 72.five 4.72* 61.four two.1* 62.5 three.2* 63.1 4.6* 63.9 six.0* 63.6 4.8* 64.4 4.1* 65.0 2.3* 66.4 three.23* 64.7 3.6* 65.eight 3.3* Vmax (v/s) 24 four.1* 19.two 3.3* 20.six three.2* 21.four six.1* 22.eight 5.3* 20.5 5.8* 23.4 four.9* 21.6 5.91* 23.six 5.1* 20.6 5.6* 21.five 3.2*of training bottle and continue which called hypoxia 1.5 h for oxygen, then to oxygen for 1 h, with normal medium nitrogen to replace the saturated medium, and continue to culture for 1 h, prepare the experiment model of hypoxia/reoxygenation. But in the course of this system, nitrogen is simple for the loss and isn’t easy to manage the content of nitrogen, and repeatability of operation is poorer. Zeng Jing etc[27] transform for no sugar serumfree media, drop 2 mL sterilization liquid paraffin wax into each well of training board, and closed for two, four, eight, 12 h, respectively, suck out paraffin wax, and wash with sterilizated of PBS for 3 instances. And after that transform for the serum containing 15 DM.
