Name :
Human MICB Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
MICA is the highly polymorphic major histocompatability complex class I chain-related protein A. MICA is a single pass type I transmembrane glycoprotein that belongs to the MIC subfamily of MHC-I family of Immunoglobulin superfamily (IgSF). MICA contains 1 Ig-like C1-type motifs (1 IgC1) in the extracellular domain (ECD) in addition to a transmembrane (TM) domain and a cytoplasmic tail. MICA is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. MICA acts as a ligand for the KLRK1/NKG2-D type II integral membrane protein receptor. Binding to KLRK1 leads to cell lysis. MICA also functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma (MM).
Gene Symbol :
NCBI Gene ID :
4277
Uniprot Entry :
Q29980
Construct Details :
The recombinant human MICB-Fc fusion is expressed as a 482 amino acid protein consisting of Glu24 – Asp266 region of MICB and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human MICB-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 EPHSLRYNLM VLSQDGSVQS GFLAEGHLDG QPFLRYDRQK RRAKPQGQWA EDVLGAETWD 70 80 90 100 110 120 TETEDLTENG QDLRRTLTHI KDQKGVPQSS RAQTLAMNVT NFWKEDAMKT KTHYRAMQAD 130 140 150 160 170 180 CLQKLQRYLK SGVAIRRTVP PMVNVTCSEV SEGNITVTCR ASSFYPRNIT LTWRQDGVSL 190 200 210 220 230 240 SHNTQQWGDV LPDGNGTYQT WVATRIRQGE EQRFTCYMEH SGNHGTHPVP SGKALVLQSQ 250 260 270 280 290 300 RTDGSTTENL YFQGSTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD 310 320 330 340 350 360 VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN 370 380 390 400 410 420 KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG 430 440 450 460 470 480 QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
M.W. :
Calculated molecular mass (kDa): 54.3; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
Calculated PI :
7.73
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 80955
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
MICB; MIC-B; PERB11.2
Research Area :
Immunology
Pathway/Disease :
NK Cell Lectin-like Receptor
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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