Name :
Human CD32B/FCGR2B/FCGRIIb Protein, ECD (Extracellular Domain), Recombinant
Description :
There are 3 classes of receptors for the Fc region of IgG (FcγRs): FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), each with multiple isoforms. All these receptors are members of the Ig superfamily (IgSF) and they play important roles in the activation or inhibition of immune responses. FcγRI is a high-affinity receptor for IgG while both FcγRII and FcγRIII are low-affinity receptors that bind IgG in the form of immune complexes. There are 3 genes for human FcγRII (CD32A, B, & C) that encodes single pass type I transmembrane proteins. The extracellular domain (ECD) of 3 human FcγRIIs shares ~90% amino acid sequence homology and contains 2 Ig-like C2-type motifs. FcγRIIs are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. CD32A and CD32C contain immunoreceptor tyrosine-based activation motifs (ITAMs) while CD32B has an inhibitory motif (ITIM) in their cytoplasmic regions. Together they act as an activating or inhibiting receptor pair to regulate immune responses.. CD32B and CD32C have almost identical ECDs. CD32B Is expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). but not detected in natural killer cells. In contrast to CD32A and CD32C, CD32B delivers an inhibitory signal.
Gene Symbol :
NCBI Gene ID :
2213
Uniprot Entry :
P31994
Construct Details :
The recombinant human CD32b ECD is expressed as a 186 amino acid protein consisting of Thr43 – Ser218 region of CD32b and a C-terminal His-tag. It contains 3 potential sites for N-linked glycosylation
Source :
Human cells stably expressing human CD32b ECD and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 TPAAPPKAVL KLEPQWINVL QEDSVTLTCR GTHSPESDSI QWFHNGNLIP THTQPSYRFK 70 80 90 100 110 120 ANNNDSGEYT CQTGQTSLSD PVHLTVLSEW LVLQTPHLEF QEGETIVLRC HSWKDKPLVK 130 140 150 160 170 180 VTFFQNGKSK KFSRSDPNFS IPQANHSHSG DYHCTGNIGY TLYSSKPVTI TVQAPSTGHH HHHHHH
M.W. :
Calculated molecular mass (kDa): 21; Estimated by SDS-PAGE under reducing condition (kDa): 30-40
Calculated PI :
6.96
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 29700
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds human IgG or Fc-fusion proteins with low affinity as measured by a functional ELISA (estimated Kd of ~100 nM)
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD32B; FCGR2B; FCG2; CD32; FCGR2; IGFR2
Research Area :
Immunology
Pathway/Disease :
Fc-Binding
Species :
Human
CD Antigen :
CD32B
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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