Name :
Human CD5/Leu1/Ly1/T1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
T-cell surface glycoprotein CD5, also known as Leu1, Ly1, and T1, is a single pass type I transmembrane protein belonging to the scavenger receptor superfamily. CD5 contains a 348 amino acid (aa) extracellular domain (ECD) with 3 scavenger receptor cysteinerich (SRCR) domains. Crystal structure of the 3rd ECD of CD5 reveals the fold of a group B SRCR domain. The ECD of CD5 can be cleaved and circulates in the serum. T cell antigen receptor (TCR) positively regulates CD5 expression on developing thymocytes and on peripheral CD4+ T cells. CD5 is known to inhibit TCR signaling and promotes T cell anergy and survival. CD5 signaling negatively regulates the generation of regulatory T cells (Treg) but promotes the development of Th17 cells. CD5 interacts with CD72/Lyb-2 on B cells and with PTPN6/SHP-1 for intracellular signaling. Within the B cell lineage, CD5 is expressed on B1a cells, anergic B cells, and IL10 producing regulatory B cells. Similar to its role on T cells, CD5 negatively regulates signaling through the B cell antigen receptor (BCR) and supports B cell survival, anergy, and tolerance. CD5 is also involved in the cellular entry of hepatitis C virus into T lymphocytes.
Gene Symbol :
NCBI Gene ID :
921
Uniprot Entry :
P06127
Construct Details :
The recombinant human CD5-Fc protein is expressed as a 576-amino acid protein consisting of Arg25 – Pro372 region of CD5 with a Fc-fusion from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD5-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 RLSWYDPDFQ ARLTRSNSKC QGQLEVYLKD GWHMVCSQSW GRSSKQWEDP SQASKVCQRL 70 80 90 100 110 120 NCGVPLSLGP FLVTYTPQSS IICYGQLGSF SNCSHSRNDM CHSLGLTCLE PQKTTPPTTR 130 140 150 160 170 180 PPPTTTPEPT APPRLQLVAQ SGGQHCAGVV EFYSGSLGGT ISYEAQDKTQ DLENFLCNNL 190 200 210 220 230 240 QCGSFLKHLP ETEAGRAQDP GEPREHQPLP IQWKIQNSSC TSLEHCFRKI KPQKSGRVLA 250 260 270 280 290 300 LLCSGFQPKV QSRLVGGSSI CEGTVEVRQG AQWAALCDSS SARSSLRWEE VCREQQCGSV 310 320 330 340 350 360 NSYRVLDAGD PTSRGLFCPH QKLSQCHELW ERNSYCKKVF VTCQDPNPST GTHTCPPCPA 370 380 390 400 410 420 PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP 430 440 450 460 470 480 REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL 490 500 510 520 530 540 PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT 550 560 570 VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
M.W. :
Calculated molecular mass (kDa): 64.2 Estimated by SDS-PAGE under reducing condition (kDa): 70-75.
Calculated PI :
8.05
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 93080
Endotoxin Level :
>92% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to human CD72 (SKU#FCL2666) measured by its binding ability in a functional ELISA.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD5; Leu-1; Ly-1; T1; LEU1; Ly1
Research Area :
Immunology
Pathway/Disease :
TCR Signaling
Species :
Human
CD Antigen :
CD5
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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