Name :
Human GPNMB/NMB/HGFIN Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
GPNMB (transmembrane glycoprotein NMB), also known as Osteoactivin and HGFIN (hematopoietic growth factor-inducible neurokinin 1 ), is a single pass type I transmembrane glycoprotein of the NMB/pMEL17 family. GPNMB is expressed in many cell types, including osteoclasts, macrophages, fibroblasts, dendritic cells, retinal pigment epithelial cells, melanocytes and tumor cells. It is often associated with the endosomal/lysosomal compartment. GPNMB is composed of a 465 aa luminal/extracellular domain (ECD), a transmembrane segment and a 53 aa cytoplasmic tail. The ECD contains a heparinbinding motif, multiple glycosylation sites, an RGD motif and an 88 aa PKD domain. The cytoplasmic tail has an ITAM (YxxI) and lysosomal targeting (LL) motif. GPNMB may paly a role in osteoblast maturation, cell adhesion and migration. GPNMB may mediate BMP-2 effects on osteoblast differentiation and function. GPNMB may also participates in bone mineralization, and acts as a negative regulator of inflammation in macrophages. GPNMB expression downregulates the macrophage inflammatory response by inhibiting IL6 and IL12 production. GPNMB is reported to bind to heparan sulfateproteoglycan and integrins in mice. GPNMB is expressed at high levels in normal and inflammatory liver macrophages implicating a role in acute liver injury. GPNMB may also play a role in triggering renal interstitial fibrosis through activation of matrix metalloproteinase expression and collagen remodeling. GPNMB is expressed in human breast cancers and is capable of promoting breast cancer.
Gene Symbol :
NCBI Gene ID :
10457
Uniprot Entry :
Q14956
Construct Details :
The recombinant human GPNMB-Fc fusion is expressed as a 693 amino acid protein consisting of Ala22 – Pro486 region of GPNMB (Uniprot Accession #Q14956) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing GPNMB-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 AKRFHDVLGN ERPSAYMREH NQLNGWSSDE NDWNEKLYPV WKRGDMRWKN SWKGGRVQAV 70 80 90 100 110 120 LTSDSPALVG SNITFAVNLI FPRCQKEDAN GNIVYEKNCR NEAGLSADPY VYNWTAWSED 130 140 150 160 170 180 SDGENGTGQS HHNVFPDGKP FPHHPGWRRW NFIYVFHTLG QYFQKLGRCS VRVSVNTANV 190 200 210 220 230 240 TLGPQLMEVT VYRRHGRAYV PIAQVKDVYV VTDQIPVFVT MFQKNDRNSS DETFLKDLPI 250 260 270 280 290 300 MFDVLIHDPS HFLNYSTINY KWSFGDNTGL FVSTNHTVNH TYVLNGTFSL NLTVKAAAPG 310 320 330 340 350 360 PCPPPPPPPR PSKPTPSLAT TLKSYDSNTP GPAGDNPLEL SRIPDENCQI NRYGHFQATI 370 380 390 400 410 420 TIVEGILEVN IIQMTDVLMP VPWPESSLID FVVTCQGSIP TEVCTIISDP TCEITQNTVC 430 440 450 460 470 480 SPVDVDEMCL LTVRRTFNGS GTYCVNLTLG DDTSLALTST LISVPSTGTH TCPPCPAPEL 490 500 510 520 530 540 LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE 550 560 570 580 590 600 QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS 610 620 630 640 650 660 REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK 670 680 690 SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK M.W.: Calculated molecular mass (kDa): 77.6; Estimated by SDS-PAGE under reducing condition (kDa): 95-100 Calculated PI: 6.08 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 120750 Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Immobilized GPNMB-Fc induces proMMP9 secretion in human normal lung fibroblasts (NHLF). FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. J. Biol. Chem. 276:8125 (2001) 2. Nat. Genet. 30:81 (2002). 3. Proc. Natl. Acad. Sci. 102:16013 (2005). 4. J. Hepatol. 42:565 (2005). 5. Clin. Cancer Res. 12:1970 (2006). 6. J. Immunol. 178:6557 (2007) Documentation Product datasheet (pdf) can be downloaded here: FCL2301-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 6.08
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized GPNMB-Fc induces proMMP9 secretion in human normal lung fibroblasts (NHLF).
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
GPNMB; NMB; HGFIN; UNQ1725/PRO9925
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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