Name :
Human CD3e Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3e, T3e, TCRe and IMD18, is a single-pass type I transmembrane protein. CD3e contains 1 Ig-like (immunoglobulin-like) domain in the extracellular region and 1 cytoplasmic ITAM (immune tyrosine activation motif) domain. CD3e, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor (TCR) alpha/beta and gamma/delta heterodimers, forms the TCR-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways leading to the activation of T cells. CD3e plays an essential role in T-cell development. In CD3e-deficient mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated. Mutations in the CD3e gene are associated with immunodeficiency 18 (IMD18), an autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood. The CD3e gene has also been linked to a susceptibility to type I diabetes in women.
Gene Symbol :
CD3e; T3E; TCRE; IMD18; Leu-4
NCBI Gene ID :
916
Uniprot Entry :
P07766
Construct Details :
The recombinant human CD3e-Fc fusion is expressed as a 333 amino acid protein consisting of Asp23 – Val127 region of CD3e (UniProt accession #P07766) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing CD3e-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL 70 80 90 100 110 120 SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMDVSTGTH TCPPCPAPEL 130 140 150 160 170 180 LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE 190 200 210 220 230 240 QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS 250 260 270 280 290 300 REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK 310 320 330 SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK M.W.: Calculated molecular mass (kDa): 37.4; Estimated by SDS-PAGE under reducing condition (kDa): ~45 Calculated PI: 5.28 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 51965 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Recognized by recombinant anti-CD3e monoclonal antibodies: human IgG1 (SKU#MAB0818), mouse IgG1 (SKU#MAB1899), mouse IgG2a (SKU#MAB1898) and rabbit IgG (SKU#MAB1897). FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Nature 321:431 (1986). 2. Nat. Genet. 3:77 (1993). 3. J. Clin. Invest. 114:1512 (2004). 4. Proc. Natl. Acad. Sci. 101:7675 (2004). 5. J Immunol. 183: 1055 (2009). Documentation Product datasheet (pdf) can be downloaded here: FCL1016-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 5.28
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Recognized by recombinant anti-CD3e monoclonal antibodies: human IgG1 (SKU#MAB0818), mouse IgG1 (SKU#MAB1899), mouse IgG2a (SKU#MAB1898) and rabbit IgG (SKU#MAB1897).
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD3e; T3E; TCRE; IMD18; Leu-4
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD3e
References :
1. Nature 321:431 (1986). 2. Nat. Genet. 3:77 (1993). 3. J. Clin. Invest. 114:1512 (2004). 4. Proc. Natl. Acad. Sci. 101:7675 (2004). 5. J Immunol. 183: 1055 (2009).
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