Name :
Human CD158B2/KIR2DL3 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
Killer cell immunoglobulin-like receptors (KIRs), also known as Killer-cell Inhibitory Receptors, are transmembrane glycoproteins that belong to the immunoglobulin superfamily (IgSF). The KIR genes are highly homologous and polymorphic. Most of them are found in a cluster on chromosome 19q13.4 within the leukocyte receptor complex (LRC). KIRs are expressed on natural killer (NK) cells and subsets of T cells. KIR proteins are composed of two or three extracellular immunoglobulin (Ig) domains, a transmembrane region and cytoplasmic tail which can in turn be short or long. The KIR proteins are thus classified by the number of Ig-domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM). In contrast KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. KIR2DL subgroup proteins, including KIR2DL1 (CD158A), KIR2DL2 (CD158B1), and KIR2DL3 (CD158B2), are receptors on NK cells for HLA-C alleles and inhibit the activity of NK cells thus preventing cell lysis.
Gene Symbol :
NCBI Gene ID :
3804
Uniprot Entry :
P43628
Construct Details :
The recombinant human KIR2DL3-Fc fusion is expressed as a 449 amino acid protein consisting of His22 – Arg242 region of KIR2DL3 (UniProt accession #P43628) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted).
Source :
Human cells stably expressing KIR2DL3-Fc growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 HEGVHRKPSL LAHPGPLVKS EETVILQCWS DVRFQHFLLH REGKFKDTLH LIGEHHDGVS 70 80 90 100 110 120 KANFSIGPMM QDLAGTYRCY GSVTHSPYQL SAPSDPLDIV ITGLYEKPSL SAQPGPTVLA 130 140 150 160 170 180 GESVTLSCSS RSSYDMYHLS REGEAHERRF SAGPKVNGTF QADFPLGPAT HGGTYRCFGS 190 200 210 220 230 240 FRDSPYEWSN SSDPLLVSVT GNPSNSWLSP TEPSSETGNP RSTGTHTCPP CPAPELLGGP 250 260 270 280 290 300 SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS 310 320 330 340 350 360 TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSREEM 370 380 390 400 410 420 TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ 430 440 QGNVFSCSVM HEALHNHYTQ KSLSLSPGK M.W.: Calculated molecular mass 49.8 kDa; estimated by SDS-PAGE under reducing condition 60-65 kDa probably due to glycosylation. Calculated PI: 6.46 Calculated Extinction Coefficient (M-1 cm-1, at 280nm): 64455 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”) Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free). Endotoxin Level: Biological Activity: Binds anti-KIR monoclonal antibodies, human IgG4 (SKU#MAB1939), human IgG1 (SKU#MAB2024) and rabbit IgG (SKU#MAB2027). FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. Storage The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN. References 1. Science 268:405 (1995) 2. Immunity 2:439 (1995) 3. Nature. 389:96 (1997) 4. Immunity 7:753 (1997) 5. Annu. Rev. Immunol. 23:225 (2005) Documentation Product datasheet (pdf) can be downloaded here: FCL2044-PDS.pdf Additional supporting documents, including COA and MSDS are available upon request.
M.W. :
: 6.46
Calculated PI :
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT “+”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds anti-KIR monoclonal antibodies, human IgG4 (SKU#MAB1939), human IgG1 (SKU#MAB2024) and rabbit IgG (SKU#MAB2027).
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
KIR2DL3; CD158B2; p58; NKAT; GL183; NKAT2; CD158b; NKAT2A; NKAT2B; KIR-K7b; KIR-K7c; KIR2DS5; KIRCL23; KIR-023GB
Research Area :
Immunology
Pathway/Disease :
NK Cell Activation
Species :
Human
CD Antigen :
CD158B2
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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