Name :
Human FLT4 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
Vascular endothelial cell growth factors (VEGFs) are secreted polypeptides with a highly conserved cystine-knot structure similar to that of the platelet-derived growth factors (PDGFs). VEGFs play important roles in vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis. VEGFs stimulate angiogenesis by binding to the specific receptors (VEGFRs) on the surface of vascular endothelial cells. All the three VEGFRs, VEGFR1 (Flt-1), VEGFR2 (KDR/Flk-1/CD309), and VEGFR3 (Flt-4), belong to the CSF-1/PDGF receptor subfamily of receptor tyrosine kinase superfamily (RTKs). Each VEGFR contains 7 immunoglobulin (Ig)-like C2-type domains in the extracellular region and an intracellular kinase domain. VEGFR3 is widely expressed in the early embryo but restricted to lymphatic vessels at later stages of development. VEGFR3 is up-regulated in tumor angiogenesis. VEGFC and VEGFD have been identified as ligands for VEGFR3. VEGFR3 plays a significant role in ligand-mediated lymphangiogenesis. Mutation in this gene is associated with the primary lymphedema, a rare genetic condition with both autosomal dominant and autosomal recessive modes of inheritance. The VEGFR3-mediated signaling pathway may provide a target for anti-lymphangiogenic therapy in cancer.
Gene Symbol :
The recombinant human FLT4-Fc fusion is expressed as a 525 amino acid protein consisting of Tyr25 – Thr323 region of FLT4 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
NCBI Gene ID :
2324
Uniprot Entry :
P35916
Construct Details :
The recombinant human FLT4-Fc fusion is expressed as a 525 amino acid protein consisting of Tyr25 – Thr323 region of FLT4 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing FLT4-Fc and growing in chemical-defined media with no animal components or antibiotics.
Amino Acid Sequence: :
10 20 30 40 50 60 YSMTPPTLNI TEESHVIDTG DSLSISCRGQ HPLEWAWPGA QEAPATGDKD SEDTGVVRDC 70 80 90 100 110 120 EGTDARPYCK VLLLHEVHAN DTGSYVCYYK YIKARIEGTT AASSYVFVRD FEQPFINKPD 130 140 150 160 170 180 TLLVNRKDAM WVPCLVSIPG LNVTLRSQSS VLWPDGQEVV WDDRRGMLVS TPLLHDALYL 190 200 210 220 230 240 QCETTWGDQD FLSNPFLVHI TGNELYDIQL LPRKSLELLV GEKLVLNCTV WAEFNSGVTF 250 260 270 280 290 300 DWDYPGKQAE RGKWVPERRS QQTHTELSSI LTIHNVSQHD LGSYVCKANN GIQRFRESTG 310 320 330 340 350 360 THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV 370 380 390 400 410 420 EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ 430 440 450 460 470 480 PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG 490 500 510 520 SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK M.W.: Calculated molecular mass (kDa): 59.1; Estimated by SDS-PAGE under reducing condition (kDa): 70-80 Calculated PI: 5.63 Calculated Extinction Coefficients(M-1 cm-1, at 280nm): 102175 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above) Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure. Endotoxin Level: Biological Activity: Binds VEGF-C and VEGF-D and inhibits VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVECs)
M.W. :
Calculated molecular mass (kDa): 59.1; Estimated by SDS-PAGE under reducing condition (kDa): 70-80
Calculated PI :
5.63
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds VEGF-C and VEGF-D and inhibits VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVECs)
Molecule Class :
Receptor Tyrosine Kinase (RTK)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
FLT4; PCL; FLT-4; LMPH1A; VEGFR3
Research Area :
Cancer
Pathway/Disease :
VEGF Signaling Pathway
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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