Name :
Human ACVR2A Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death. The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal. Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. ACVR2A (Activin receptor type-2A), also known as ACVR2 and ACTRIIA, is a type II receptors for Activins and Inhibins as well as several other members of the TGFβ superfamily proteins. Following the binding to dimeric Activin ligands, ACVRIIA then associates with a type I receptor to initiate signal transduction. Human, mouse and rat ACVRIIA share greater than 98% amino acid sequence homology. Recombinant soluble ACVRIIA bind activin with high affinity, and are potent activin antagonists. ACVR2A may function as a tumor suppressor that is frequently mutated in microsatellite-unstable colon cancers. Inactivation of ACVR2A is also a common event in prostate cancer cells. ACVR2A may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality.
Gene Symbol :
ACVR2A; ACVR2; ACTRII; ActR-II; ACTRIIA; ActR-IIA; ACVRIIA; ACVR-IIA
NCBI Gene ID :
92
Uniprot Entry :
P27037
Construct Details :
The recombinant human ACVR2A-Fc fusion protein is expressed as a 344 amino acid protein consisting of Ala20 – Pro135 region of ACVR2A (UniProt accession #P27037) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image above, labeled as “DTT: -“)
Source :
Human cells stably expressing human ACVR2A-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
AILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGS IEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEME VTQPTSNPVTPKPPSTGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRT PEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLT VLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEM TKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 39.0; Estimated by SDS-PAGE under reducing condition (kDa): ~55
Calculated PI :
5.97
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 58870
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized ACVR2A binds Activin or Inhibin in a functional ELISA. Neutralize Activin-induced inhibition of MPC11 cell proliferation and hemoglobin expression in K562 human chronic myelogenous leukemia cells.
Molecule Class :
Serine/Threonine Kinase Receptor
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
ACVR2; ACTRII; ActR-II; ACTRIIA; ActR-IIA; ACVRIIA; ACVR-IIA
Research Area :
Development
Pathway/Disease :
Activin/TGFβ Signaling Pathway
Species :
Human
CD Antigen :
References :
1. Nature 401:480 (1999). 2. J. Biol. Chem. 274:584 (1999). 3. Nature 404:411 (2000). 4. Proc. Natl. Acad. Sci. 100:5193 (2003). 5. Cytokine Growth Factor Rev. 17:157 (2006).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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