Name :
Mouse PD-L2 protein, ECD (extracellular domain), Fc-fusion, recombinant
Description :
PD-L2 (programmed death ligand 2), also referred to as B7-DC or CD273, is a single pass type I transmembrane glycoprotein that belongs to the B7 family of the Ig superfamily. It was originally identified as a homolog of PD-L1. Like PD-L1 and other B7 family members, PD-L2 contains one Ig V-like and one Ig C-like domain in the extracellular region. Among the family members, they share about 20-25% amino acid identity. PDL1 and PDL2 share ~41% amino acid sequence identity and have similar functions. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS and INF-γ. PD-L2 and PD-L1 are two ligands for PD-1/CD279, a member of the CD28/CTLA4 family receptors that play critical roles in regulating T cell activation and immune tolerance. Interaction with PD-1, which has a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), inhibits T-cell proliferation and cytokine production. PD-L2 is also a dendritic cell molecule with co-stimulatory properties for T cells. The interaction of PD-L2/PD-1 has a 2-6-fold higher affinity than that of B7-H1/PD-1.
Gene Symbol :
The recombinant mouse PD-L2-Fc fusion is expressed as a 438 amino acid protein consisting of Leu20 – Thr220 region of mouse PD-L2 (UniProt accession #Q9WUL5) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
NCBI Gene ID :
58205
Uniprot Entry :
Q9WUL5
Construct Details :
The recombinant mouse PD-L2-Fc fusion is expressed as a 438 amino acid protein consisting of Leu20 – Thr220 region of mouse PD-L2 (UniProt accession #Q9WUL5) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing mouse PD-L2-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 FTVTAPKEVY TVDVGSSVSL ECDFDRRECT ELEGIRASLQ KVENDTSLQS ERATLLEEQL 70 80 90 100 110 120 PLGKALFHIP SVQVRDSGQY RCLVICGAAW DYKYLTVKVK ASYMRIDTRI LEVPGTGEVQ 130 140 150 160 170 180 LTCQARGYPL AEVSWQNVSV PANTSHIRTP EGLYQVTSVL RLKPQPSRNF SCMFWNAHMK 190 200 210 220 230 240 ELTSAIIDPL SRMEPKVPRT STTENLYFQG STGTHTCPPC PAPELLGGPS VFLFPPKPKD 250 260 270 280 290 300 TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL 310 320 330 340 350 360 HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV 370 380 390 400 410 420 KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH 430 EALHNHYTQK SLSLSPGK
M.W. :
Calculated molecular mass 49.2 kDa; estimated by SDS-PAGE under reducing condition ~60 kDa (probably due to glycosylation)
Calculated PI :
6.86
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its receptor PD-1 in a functional ELISA. Blocks the ligand PD-L1’s and PD-L2’s binding to its receptor and mediated signaling activity. Shows cross-species binding to human PD-1 (SKU# FCL0763B) with the highest affinity among all human and murine PD-1 ligands (see Technical Data).
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD273; B7-DC; PDL2; PDCD1LG2; B7DC; CD273; PDCD1L2; PDL2
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Mouse
CD Antigen :
CD273
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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