Name :
Human CD27 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD27 is a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF7). CD27 is expressed as a disulfidelinked homodimer on the cell surface. Proteolytic cleavage of CD27 results in the shedding of a 2832 kDa fragment of the extracellular domain (ECD) with three TNFR cysteinerich repeats. The expression of CD27 is limited to cells of the lymphoid lineage. CD27 is weakly expressed on naïve T cells and NK cells and is upregulated upon cell activation. It is also upregulated on activated germinal center B cells, plasma cells, and a subset of memory B cells. CD27 acts as a costimulatory molecule that supports lymphocyte activation and survival. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by a TNF-like ligand CD70 on lymphocytes and dendritic cells. CD27 binds to the transmembrane glycoprotein CD70 that is expressed on activated B cells, activated T cells, and dendritic cells. CD27 transduces signals and subsequently leads to the activation of NF-κB and JNK pathways that contribute to the activation and survival of T cells and NK cells as well as the long-term maintenance of T cell immunity. CD27 also plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses. Mutations in the CD27 gene are associated with an autosomal recessive immunodeficiency disorder, lymphoproliferative syndrome 2 (LPFS2).
Gene Symbol :
TNFRSF7; T14; S152; Tp55; S152. LPFS2
NCBI Gene ID :
939
Uniprot Entry :
P26842
Construct Details :
The recombinant human CD27-Fc fusion is expressed as a 400 amino acid protein consisting of Ala20 – Arg191 region of CD27 (UniProt accession #P26842) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing human CD27-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 ATPAPKSCPE RHYWAQGKLC CQMCEPGTFL VKDCDQHRKA AQCDPCIPGV SFSPDHHTRP 70 80 90 100 110 120 HCESCRHCNS GLLVRNCTIT ANAECACRNG WQCRDKECTE CDPLPNPSLT ARSSQALSPH 130 140 150 160 170 180 PQPTHLPYVS EMLEARTAGH MQTLADFRQL PARTLSTHWP PQRSLCSSDF IRSTGTHTCP 190 200 210 220 230 240 PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA 250 260 270 280 290 300 KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ 310 320 330 340 350 360 VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY 370 380 390 400 SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 44.8; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
Calculated PI :
7.81
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 56265
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand CD70 and inhibits CD70-mediated signaling activities including proliferation of T cells induced by CD70 and antiCD3 antibody.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD27; TNFRSF7; T14; S152; Tp55; S152. LPFS2
Research Area :
Immunology
Pathway/Disease :
T Cell Costimulation
Species :
Human
CD Antigen :
CD27
References :
1. J. Immunol. 139:1589 (1987). 2. Cell 73:447 (1993). 3. J. Immunol. 152:1756 (1994). 4. Proc. Natl. Acad. Sci. 92: 11249 (1995). 5. Immunol. Rev. 229:216 (2009).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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