Name :
Human TNFR2 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
TNFR1 and TNFR2 are single-pass, type I transmembrane glycoproteins that are two distinct types of highaffinity cell surface receptors for TNF. Both receptors are the founding members of the TNF receptor superfamily (TNFRSF) and are designated TNFRSF1A and TNFRSF1B, respectively. The extracellular regions of both receptors contain 4 conserved cysteinerich repeats that mediate the interaction with TNF. However their intracellular sequences have no homology, indicative of distinct signaling pathways. TNFR1 and TNFR2 form a heterocomplex on the cell surface that mediates the recruitment of adaptor proteins for signal transduction. Soluble truncated forms of TNFR1 and TNFR2 have been identified in human serum and urine, which can neutralize the biological activities of TNF. Whereas all cell types studied express TNFR1, TNFR2 expression is limited primarily to hematopoietic cells and cells of the immune system. TNFR2 mediates most of the metabolic effects of TNF. TNFR2 binds with high affinity to TNF-α and approximately 5-fold lower affinity to TNF-β. The soluble form of TNFR2 is produced from the membrane form by proteolytic processing and is clinically used to treat moderate to severe rheumatoid arthritis (RA). In addition high plasma levels of soluble TNFR2 are associated with increased incidence of coronary heart disease.
Gene Symbol :
TNFRSF1B; CD120b; p75; TBPII; TNFBR; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-RII
NCBI Gene ID :
7133
Uniprot Entry :
P20333
Construct Details :
The recombinant human TNFR2-Fc fusion is expressed as a 460 amino acid protein consisting of Leu23 – Asp257 region of TNFR2 (UniProt accession #P20333) and a C-terminal Fc from human IgG1, which exists as a dimer/tetramer under non-reducing conditions (see the gel image above).
Source :
Human cells stably expressing human TNFR2-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 LPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST 70 80 90 100 110 120 YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK 130 140 150 160 170 180 CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS 190 200 210 220 230 240 TSPTRSMAPG AVHLPQPVST RSQHTQPTPE PSTAPSTSFL LPMGPSPPAE GSTGDTHTCP 250 260 270 280 290 300 PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA 310 320 330 340 350 360 KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ 370 380 390 400 410 420 VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY 430 440 450 460 SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 50.5; Estimated by SDS-PAGE under reducing condition (kDa): 75-85
Calculated PI :
7.15
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 61110
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Binds to its ligand TNF and inhibits TNF-mediated biological activities including NF-κB activation and cytotoxicity in the mouse L929 fibrosarcoma cells.
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TNFRSF1B; CD120b; p75; TBPII; TNFBR; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-RII
Research Area :
Immunology
Pathway/Disease :
TNF Signaling Pathway
Species :
Human
CD Antigen :
CD120b
References :
1. Science 248:1019 (1990). 2. Cell 78:681-692(1994) 3. Cell 104:487 (2001). 4. Annu. Rev. Immunol. 19:163 (2001). 5. Science 296:1634 (2002).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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