Name :
Human ROR1 Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
ROR1 (receptor tyrosine kinase-like orphan receptor 1), also known as NTRKR1 (neurotrophic tyrosine kinase, receptor-related 1), is a single-pass type I transmembrane glycoprotein that belongs to the ROR subfamily of the tyrosine protein kinase family. The ROR subfamily members, ROR1 and ROR2, are tyrosine kinase receptors important in regulating skeletal and neuronal development, cell migration and cell polarity. Each ROR member contains 1 FZ (frizzled) domain, 1 Ig (immunoglobulin)-like C2-type domain, and 1 kringle domain in the extracellular region and 1 protein kinase domain in the cytoplasmic region. ROR1 is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with various hematological malignancies of both lymphoid and myeloid origins as well as in melanoma. ROR1 is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. ROR1 modulates neurite growth in the central nervous system. ROR1 may play a role in the survival of melanoma cells and regulate EMT and tumor metastasis.
Gene Symbol :
NTRKR1; dJ537F10.1
NCBI Gene ID :
4919
Uniprot Entry :
Q01973
Construct Details :
The recombinant human ROR1-Fc fusion is expressed as a 602 amino acid protein consisting of Gln30 – Glu403 region of ROR1 (Uniprot #Q01973) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).
Source :
Human cells stably expressing ROR1-Fc and growing in chemical-defined media with no animal components or antibiotics.
Amino Acid Sequence: :
10 20 30 40 50 60 QETELSVSAE LVPTSSWNIS SELNKDSYLT LDEPMNNITT SLGQTAELHC KVSGNPPPTI 70 80 90 100 110 120 RWFKNDAPVV QEPRRLSFRS TIYGSRLRIR NLDTTDTGYF QCVATNGKEV VSSTGVLFVK 130 140 150 160 170 180 FGPPPTASPG YSDEYEEDGF CQPYRGIACA RFIGNRTVYM ESLHMQGEIE NQITAAFTMI 190 200 210 220 230 240 GTSSHLSDKC SQFAIPSLCH YAFPYCDETS SVPKPRDLCR DECEILENVL CQTEYIFARS 250 260 270 280 290 300 NPMILMRLKL PNCEDLPQPE SPEAANCIRI GIPMADPINK NHKCYNSTGV DYRGTVSVTK 310 320 330 340 350 360 SGRQCQPWNS QYPHTHTFTA LRFPELNGGH SYCRNPGNQK EAPWCFTLDE NFKSDLCDIP 370 380 390 400 410 420 ACDSKDSKEK NKMESTGTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD 430 440 450 460 470 480 VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN 490 500 510 520 530 540 KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG 550 560 570 580 590 600 QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK M.W.: Calculated molecular mass (kDa): 67.5; Estimated by SDS-PAGE under reducing condition (kDa): 75-85 Calculated PI: 5.90 Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 79770 Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above) Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free). Endotoxin Level: Biological Activity: Not Available
M.W. :
Calculated molecular mass (kDa): 67.5; Estimated by SDS-PAGE under reducing condition (kDa): 75-85
Calculated PI :
5.90
Calculated Extinction Coefficients :
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Not Available
Molecule Class :
Receptor Tyrosine Kinase (RTK)
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
NTRKR1; dJ537F10.1
Research Area :
Neuroscience
Pathway/Disease :
Wnt Signaling Pathway
Species :
Human
CD Antigen :
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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