Name :
Human NRP1/CD304/NRP/BDCA4/VEGF165R/neuropilin1 Protein, ECD (Extracellular Domain), Biotinylated, Recombinant
Description :
NRP1 (neuropilin-1), one of the 2 members of the neuropilin family, is a single-pass type I transmembrane glycoprotein. Both members of neuropilins contain a large N-terminal extracellular region consisting of 2 complement-binding (CUB), 2 coagulation factor V/VIII (F5/8 type C), and 1 meprin (MAM) domains in addition to a transmembrane domain and a small cytoplasmic tail. These specific domains allow neuropilins to participate in several different types of signaling pathways that control cell survival, migration, and attraction. Neuropilins bind many ligands and various types of co-receptors including vascular endothelial growth factor (VEGF) and semaphoring (SEMA) family members. The tandem CUB domains mediate the binding to SEMA, while the tandem F5/8 type C domains are responsible for heparin and VEGF binding. NRP1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. NRP1 mediates the chemorepulsant activity of semaphorins. NRP1 binds to SEMA3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. NRP1 may regulate VEGF-induced angiogenesis. The soluble isoform of NRP1 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165 and bind various members of the SEMA family. Its expression has an adverse effect on blood vessel number and integrity.
Gene Symbol :
Lys852 region of NRP1 and a C-terminal His-tag. NRP1 ECD contains 5 potential N-linked glycosylation sites.
NCBI Gene ID :
8829
Uniprot Entry :
O14786
Construct Details :
The recombinant human NRP1 ECD protein is expressed as an 842-amino acid protein consisting of Phe22 – Lys852 region of NRP1 and a C-terminal His-tag. NRP1 ECD contains 5 potential N-linked glycosylation sites.
Source :
Human cells stably expressing NRP1 ECD and growing in chemical-defined media with no animal component or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 FRNDKCGDTI KIESPGYLTS PGYPHSYHPS EKCEWLIQAP DPYQRIMINF NPHFDLEDRD 70 80 90 100 110 120 CKYDYVEVFD GENENGHFRG KFCGKIAPPP VVSSGPFLFI KFVSDYETHG AGFSIRYEIF 130 140 150 160 170 180 KRGPECSQNY TTPSGVIKSP GFPEKYPNSL ECTYIVFVPK MSEIILEFES FDLEPDSNPP 190 200 210 220 230 240 GGMFCRYDRL EIWDGFPDVG PHIGRYCGQK TPGRIRSSSG ILSMVFYTDS AIAKEGFSAN 250 260 270 280 290 300 YSVLQSSVSE DFKCMEALGM ESGEIHSDQI TASSQYSTNW SAERSRLNYP ENGWTPGEDS 310 320 330 340 350 360 YREWIQVDLG LLRFVTAVGT QGAISKETKK KYYVKTYKID VSSNGEDWIT IKEGNKPVLF 370 380 390 400 410 420 QGNTNPTDVV VAVFPKPLIT RFVRIKPATW ETGISMRFEV YGCKITDYPC SGMLGMVSGL 430 440 450 460 470 480 ISDSQITSSN QGDRNWMPEN IRLVTSRSGW ALPPAPHSYI NEWLQIDLGE EKIVRGIIIQ 490 500 510 520 530 540 GGKHRENKVF MRKFKIGYSN NGSDWKMIMD DSKRKAKSFE GNNNYDTPEL RTFPALSTRF 550 560 570 580 590 600 IRIYPERATH GGLGLRMELL GCEVEAPTAG PTTPNGNLVD ECDDDQANCH SGTGDDFQLT 610 620 630 640 650 660 GGTTVLATEK PTVIDSTIQS EFPTYGFNCE FGWGSHKTFC HWEHDNHVQL KWSVLTSKTG 670 680 690 700 710 720 PIQDHTGDGN FIYSQADENQ KGKVARLVSP VVYSQNSAHC MTFWYHMSGS HVGTLRVKLR 730 740 750 760 770 780 YQKPEEYDQL VWMAIGHQGD HWKEGRVLLH KSLKLYQVIF EGEIGKGNLG GIAVDDISIN 790 800 810 820 830 840 NHISQEDCAK PADLDKKNPE IKIDETGSTP GYEGEGEGDK NISRKPGNVL KSTGHHHHHH HH
M.W. :
Calculated molecular mass (kDa): 94.7; Estimated by SDS-PAGE under reducing condition (kDa): 100-110
Calculated PI :
5.84
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 146775
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
NRP1; NP1; NRP; BDCA4; CD304; VEGF165R; neuropilin-1; neuropilin1
Research Area :
Cancer
Pathway/Disease :
Angiogenesis
Species :
Human
CD Antigen :
CD304
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
TCN2/Transcobalamin-2 Protein
Coagulation Factor XIV/PROC Protein
Popular categories:
Death Receptor 5
APRIL Proteins
