Name :
Human Nectin2/CD112/PRR2/PVRL2 Protein, Extracellular Domain (ECD), Biotinylated, Recombinant
Description :
Nectin2 or CD112 is a single pass type I transmembrane glycoprotein that belongs to the Nectin family of the Immunoglobulin superfamily (IgSF). Most Nectin family members function as cell adhesion molecules (CAMs) located on the cell surface and involved with the binding with other cells or with the extracellular matrix (ECM). Nectin2 contains 1 Ig-like V-type domain and 2 Ig-like C2-type domains in the extracellular region that promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN2 and NECTIN3. It binds with low affinity to TIGIT. Nectin2 may modulate T-cell signaling, acting as either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, Nectin 2 stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, Nectin 2 inhibits T-cell proliferation. Nectin2 functions as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV) envelope glycoprotein D. Variations in the Nectin2 gene are associated with differences in the severity of multiple sclerosis (MS).
Gene Symbol :
Leu360 region of Nectin2 isoform alpha (UniProt #Q92692-2) and a C-terminal His-tag. Nectin2 ECD contains 2 potential N-linked glycosylation sites.
NCBI Gene ID :
5819
Uniprot Entry :
Q92692
Construct Details :
The recombinant human Nectin2/CD112 ECD protein is expressed as a 340-amino acid active form consisting of Gln32 – Leu360 region of Nectin2 isoform alpha (UniProt #Q92692-2) and a C-terminal His-tag. Nectin2 ECD contains 2 potential N-linked glycosylation sites.
Source :
Human cells stably expressing Nectin2 ECD and growing in chemical-defined media with no animal component or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QDVRVQVLPE VRGQLGGTVE LPCHLLPPVP GLYISLVTWQ RPDAPANHQN VAAFHPKMGP 70 80 90 100 110 120 SFPSPKPGSE RLSFVSAKQS TGQDTEAELQ DATLALHGLT VEDEGNYTCE FATFPKGSVR 130 140 150 160 170 180 GMTWLRVIAK PKNQAEAQKV TFSQDPTTVA LCISKEGRPP ARISWLSSLD WEAKETQVSG 190 200 210 220 230 240 TLAGTVTVTS RFTLVPSGRA DGVTVTCKVE HESFEEPALI PVTLSVRYPP EVSISGYDDN 250 260 270 280 290 300 WYLGRTDATL SCDVRSNPEP TGYDWSTTSG TFPTSAVAQG SQLVIHAVDS LFNTTFVCTV 310 320 330 340 TNAVGMGRAE QVIFVRETPR ASPRDVGPLS TGHHHHHHHH
M.W. :
Calculated molecular mass (kDa): 36.9; Estimated by SDS-PAGE under reducing condition (kDa): 40-45
Calculated PI :
5.95
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 42315
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
Nectin2; CD112; HVEB; PRR2; PVRL2; PVRR2
Research Area :
Cancer
Pathway/Disease :
Cell Adhesion
Species :
Human
CD Antigen :
CD112
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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