Name :
Human TGFBR3/BGCAN Protein, ECD (Extracellular Domain), Recombinant
Description :
The TGFβ (transforming growth factor β) superfamily proteins are pleiotropic cytokines that regulate a diverse range of cellular processes, including proliferation, differentiation, migration, adhesion and death. The TGFβ superfamily elicits 4 signaling pathways: TGFβ, Bone Morphogenic Protein (BMP), Activin, and Nodal. Each pathway signals through a heteromeric receptor complex composed of at least two type-I and two type-II transmembrane receptors containing cytoplasmic serine/threonine kinase domains. These receptors are distinguished by the presence of a glycine/serine-rich juxta-membrane domain found only in the type I receptors. Either type receptor may initially bind ligand, followed by the recruitment of an alternate-type receptor counterpart to form a signal-activating complex. TGFBR3 (TGFβ receptor 3), also known as BGCAN and Betaglycan, is a transmembrane proteoglycan with a large extracellular domain and a short cytoplasmic domain that lacks an obvious signaling motif. TGFR3 functions as a co-receptor with other TGFBR superfamily members. It may be involved in capturing and retaining the ligand for presentation to the signaling receptors. TGFBR3 binds TGFβ2 with the highest affinity and other TGFβ isoforms with lower affinities. Cellular responsiveness to TGFβ2 is dependent on the presence of TGFBR3. Ectodomain shedding produces a soluble form of TGFBR3, which binds TGFβ and exhibits antagonistic activity. Reduced expression of TGFBR3 has been observed in various cancers. Genetic variations in TGFBR3 are associated with Vogt-Koyanagi-Harada (VKH) disease and Behcet’s disease (BD).
Gene Symbol :
TGFBR3; TGFBRIII; TGFBR-3; TGFR-3; TGFR-III; BGCAN; betaglycan
NCBI Gene ID :
7049
Uniprot Entry :
Q03167
Construct Details :
The recombinant human TGFBR3/BGCAN ECD protein is expressed as a 772 amino acid protein consisting of Gly21 – Gly781 region of TGFBR3 (UniProt accession #Q03167) and a C-terminal His tag.
Source :
Human cells stably expressing human TGFBR3 and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
GPEPGALCELSPVSASHPVQALMESFTVLSGCASRGTTGLPQEVHVLNLRTAGQGPGQLQ REVTLHLNPISSVHIHHKSVVFLLNSPHPLVWHLKTERLATGVSRLFLVSEGSVVQFSSA NFSLTAETEERNFPHGNEHLLNWARKEYGAVTSFTELKIARNIYIKVGEDQVFPPKCNIG KNFLSLNYLAEYLQPKAAEGCVMSSQPQNEEVHIIELITPNSNPYSAFQVDITIDIRPSQ EDLEVVKNLILILKCKKSVNWVIKSFDVKGSLKIIAPNSIGFGKESERSMTMTKSIRDDI PSTQGNLVKWALDNGYSPITSYTMAPVANRFHLRLENNAEEMGDEEVHTIPPELRILLDP GALPALQNPPIRGGEGQNGGLPFPFPDISRRVWNEEGEDGLPRPKDPVIPSIQLFPGLRE PEEVQGSVDIALSVKCDNEKMIVAVEKDSFQASGYSGMDVTLLDPTCKAKMNGTHFVLES PLNGCGTRPRWSALDGVVYYNSIVIQVPALGDSSGWPDGYEDLESGDNGFPGDMDEGDAS LFTRPEIVVFNCSLQQVRNPSSFQEQPHGNITFNMELYNTDLFLVPSQGVFSVPENGHVY VEVSVTKAEQELGFAIQTCFISPYSNPDRMSHYTIIENICPKDESVKFYSPKRVHFPIPQ ADMDKKRFSFVFKPVFNTSLLFLQCELTLCTKMEKHPQKLPKCVPPDEACTSLDASIIWA MMQNKKTFTKPLAVIHHEAESKEKGPSMKEPNPISPPIFHGSTGHHHHHHHH
M.W. :
Calculated molecular mass (kDa): 85.7; Estimated by SDS-PAGE under reducing condition (kDa): 95-100
Calculated PI :
5.68
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 68715
Endotoxin Level :
>75% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “DTT: +”)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Immobilized TGFBR3 protein binds human TGFβ2 in a functional ELISA. Blocks TGFβ2-mediated signaling activity.
Molecule Class :
Membrane Proteoglycan
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
TGFBRIII; TGFBR-3; TGFR-3; TGFR-III; BGCAN; betaglycan
Research Area :
Development
Pathway/Disease :
TGFβ Signaling Pathway
Species :
Human
CD Antigen :
References :
1. Biochem. Biophys. Res. Commun. 189:356 (1992). 2. J. Hum. Genet. 45:250 (2000). 3. EMBO J. 20 (3): 480-90 (2001). 4. Oncol. Rep. 30:1301-8 (2013). 5. Curr. Eye Res. 37:312 (2012).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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