Name :
Human CD138/SDC1/syndecan Protein, ECD (Extracellular Domain), Fc-fusion, Recombinant
Description :
CD138, also known as SDC1 (syndecan 1), is a transmembrane (type I) heparan sulfate proteoglycan of the syndecan proteoglycan family. The syndecans mediate cell adhesion, signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. CD138 is a 310 amino acid (aa) single pass type I transmembrane protein with an N-terminal signal peptide, a 232-aa extracellular domain (ECD) with 6 potential glycosylation sites, a transmembrane domain (TM), and a short 35-aa cytoplasmic tail. CD138 is a cell surface proteoglycan with both heparan sulfate and chondroitin sulfate. CD138 also regulates exosome biogenesis. CD138 shedding is enhanced by a number of factors such as heparanase, thrombin, PMA or EGF, and also by stress and wound healing. CD138 may play a role in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types.
Gene Symbol :
Gln23 – Glu251 region of CD138 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
NCBI Gene ID :
6382
Uniprot Entry :
P18827
Construct Details :
The recombinant human CD138-Fc is expressed as a 462-amino acid protein consisting of Gln23 – Glu251 region of CD138 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD138-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 QPALPQIVAT NLPPEDQDGS GDDSDNFSGS GAGALQDITL SQQTPSTWKD TQLLTAIPTS 70 80 90 100 110 120 PEPTGLEATA ASTSTLPAGE GPKEGEAVVL PEVEPGLTAR EQEATPRPRE TTQLPTTHLA 130 140 150 160 170 180 STTTATTAQE PATSHPHRDM QPGHHETSTP AGPSQADLHT PHTEDGGPSA TERAAEDGAS 190 200 210 220 230 240 SQLPAAEGSG EQDFTFETSG ENTAVVAVEP DRRNQSPVDQ GATGASQGLL DRKESTGTHT 250 260 270 280 290 300 CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH 310 320 330 340 350 360 NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE 370 380 390 400 410 420 PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF 430 440 450 460 LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
M.W. :
Calculated molecular mass (kDa): 49.7; Estimated by SDS-PAGE under reducing condition (kDa): 55-85
Calculated PI :
4.90
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 41285
Endotoxin Level :
>90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as “S” and “M” for marker)
Formulation :
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
SDC1; SDC; CD138; SYND1; syndecan
Research Area :
Cancer
Pathway/Disease :
Leukocyte Migration
Species :
Human
CD Antigen :
CD138
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Animal-Free IFN alpha 1a Protein
TSLPR Protein
Popular categories:
E2 Enzymes
Siglec-15
