Name :
Human CD16A/FCGR3A/FCGRIIIa F176 Protein, ECD (Extracellular Domain), Fc-fusion, Biotinylated, Recombinant
Description :
There are 3 classes of receptors for the Fc region of IgG (FcγRs): FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), each with multiple isoforms. All these receptors are members of the Ig superfamily (IgSF) and they play important roles in the activation or inhibition of immune responses. FcγRI is a high-affinity receptor for IgG while both FcγRII and FcγRIII are low-affinity receptors that bind IgG in the form of immune complexes. There are two genes for human FcγRIII: A (CD16A) that encodes a single pass transmembrane receptor and B (CD16B) that encodes a GPI-anchored protein. The extracellular domains (ECDs) of CD16A and CD16B share 97% amino acid sequence homology. CD16A is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells. Signaling through CD16A results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells as well as clearance of immune complexes. In humans, a single nucleotide polymorphism creates high Fc-binding (176V) and low Fc-binding (176F) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. CD16A surface expression requires interaction of an accessory chain, either the common gamma-chain or CD3zeta. A soluble form of CD16A can be produced by proteolytic cleavage and circulates in plasma, which is increased in rheumatoid arthritis and in coronary artery diseases.
Gene Symbol :
NCBI Gene ID :
2214
Uniprot Entry :
P08637
Construct Details :
The recombinant human CD16A-Fc is expressed as a 420-amino acid protein consisting of Gly17 – Gln208 region of CD16A-F176 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.
Source :
Human cells stably expressing human CD16A-Fc and growing in chemical-defined media with no animal components or antibiotics
Amino Acid Sequence: :
10 20 30 40 50 60 GMRTEDLPKA VVFLEPQWYR VLEKDSVTLK CQGAYSPEDN STQWFHNESL ISSQASSYFI 70 80 90 100 110 120 DAATVDDSGE YRCQTNLSTL SDPVQLEVHI GWLLLQAPRW VFKEEDPIHL RCHSWKNTAL 130 140 150 160 170 180 HKVTYLQNGK GRKYFHHNSD FYIPKATLKD SGSYFCRGLF GSKNVSSETV NITITQGLAV 190 200 210 220 230 240 STISSFFPPG YQSTGTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS 250 260 270 280 290 300 HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA 310 320 330 340 350 360 LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP 370 380 390 400 410 420 ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
M.W. :
Calculated molecular mass (kDa): 47.4; Estimated by SDS-PAGE under reducing condition (kDa): 50-55
Calculated PI :
6.74
Calculated Extinction Coefficients :
(M-1 cm-1, at 280nm): 76945
Endotoxin Level :
>95% judged by SDS-PAGE under reducing condition (see the gel image above)
Formulation :
Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.
Endotoxin Level :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Biological Activity :
Molecule Class :
1-Pass Type I Transmembrane
Gene Synonym :
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
Gene Family :
CD16A; FCGR3A; FCG3; CD16; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Research Area :
Immunology
Pathway/Disease :
Fc-Binding
Species :
Human
CD Antigen :
CD16A-176F
References :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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