Pplying the CL (E). EG-VEGF and VEGF expression (C) are weak or absent in the granulosa lutein cell layer. Note that VEGF is clearly expressed in this sample inside the vascular smooth muscle of some smaller arterioles supplying the CL (arrowheads, C). VEGFR-2 (KDR) expression is still robust in vessels in all layers of your CL (G). Scale bars: 5 mm (B); one hundred m (C); 50 m (J). GL, granulosa lutein; TL, theca lutein.pattern represent a later stage of preovulatory follicle than illustrated in Cathepsin K Proteins Source Figure 1, G to I, or regardless of whether they represent an early stage of follicular atresia. CL derived from ovulatory follicles IL-2 Inducible T-Cell Kinase (ITK/TSK) Proteins Purity & Documentation mature inside a canonical 14-day pattern.29 We examined EG-VEGF and VEGF expression inside a series of CL representing time points 2 days to 14 days right after ovulation. To convey a sense of the overall distribution of EG-VEGF and VEGF expression in individual ovaries, autoradiographic film final results of parallel sections were digitized and also the pictures corresponding to EG-VEGF and VEGF signals from representative ovarysamples were false-colored green and red, respectively. At 2 to 3 days immediately after ovulation (Figure 2; time points are inferred, as outlined by the histological criteria of Corner29), the EG-VEGF and VEGF expression resemble the pattern observed within the late preovulatory follicle: granulosa cells are intensely VEGF-positive, but lack significant EGVEGF expression (Figure two; C to F). At 5 days following ovulation (Figure three), each VEGF (Figure 3, C and D) and EG-VEGF (Figure 3, E and F) are strongly expressed in a portion of granulosa lutein cells (theca lutein cells are not clearly distinct histologically at this stage; they might alsoVEGF and EG-VEGF in Human Ovaries 1887 AJP June 2003, Vol. 162, No.Figure five. EG-VEGF and VEGF expression in normal ovary late-regressing CL. A regressing CL (around day 14 after ovulation), characterized by massive, pale, vacuolated theca granulosa and theca lutein cells (I, J), shows absence of both VEGF (C) and EG-VEGF (E) expression. A: False-colored autoradiographic film outcomes show absence of VEGF (red) and EG-VEGF (green) signal in an region that microscopically corresponds towards the regressing CL. Only weak VEGFR-2 (KDR) expression (G) is noted in scattered vessels within the granulosa cell layer. A creating tertiary (antral) follicle (A and B, arrowhead) shows robust VEGF expression (see Figure 1 for specifics). Scale bars: five mm (B); one hundred m (C); 50 m (J).express EG-VEGF and VEGF). At eight days just after ovulation (Figure four), EG-VEGF expression is intense in the theca lutein cells (Figure 4, E and F), although VEGF expression has diminished for the point exactly where only weak signal remains in the peripheral thecal cells (Figure four, C and D). Figure 5 illustrates a CL undergoing involutional alterations (approximately day 14 immediately after ovulation). Primarily no VEGF signal is present at this stage (Figure 5; A, C, and D), and EG-VEGF expression is virtually completely abolished in theca lutein cell layer (Figure five; A, E, and F).As noted in Figures four to 7, EG-VEGF expression is regularly expressed within the ovarian stroma amongst follicles, typically at reduce levels than within the theca immediately surrounding follicles, in agreement with our earlier report.18 Close to the ovarian hilum, particularly robust EG-VEGF expression is detected in clusters of cells constant with Leydig-like hilus cells30 (Figure six). As has been previously described,31,32 these cells typically occur in intimate association with blood vessels and unmyelinated nerves (Figure 6A, closed arrowhead.
