Roteins with direct or indirect association with cell junctions and/or the cytoskeleton (Table 1); (ii) seven proteins in the secretory pathway; (iii) 4 mitochondrial proteins; (iv) two chaperone proteins; and (v) one particular nucleuscytoplasm shuttling protein. Two proteins in the mitochondrion group and a single protein in the secretory pathway have been detected at the plasma membrane. For that reason, 15 out of 26 proteins may be directly or indirectly related with all the plasma membrane (12 from cell junctions or the cytoskeleton, two in the mitochondrial group, and one particular in the secretory group). A general subcellular classification in the 26 proteins is illustrated in Figure 1A.Int. J. Mol. Sci. 2018, 19,3 ofInt. J. Mol. Sci. 2018, 19, x FOR PEER REVIEW3 ofFigure 1. (A) Venn diagram representing the distribution of 26 candidate proteins to interact with Figure 1. (A) Venn diagram representing the distribution of 26 candidate proteins to interact with CX26. CX26. The subcellular classification plus the quantity of proteins in each and every of the 5 distinct groups are The subcellular classification along with the quantity of proteins in every single on the 5 distinct groups are indicated indicated also as their overlapping sorting; (B) The network of proteinprotein interaction (PPI) is also as their overlapping sorting; (B) The network of proteinprotein interaction (PPI) is predicted predicted at http://stringdb.org. Acronyms are as outlined by the genes listed on Table 1 in addition to at http://stringdb.org. Acronyms are based on the genes listed on Table 1 as well as actin B actin B (ACTB), (TUBA1A), and arginine succinate synthase 1 (ASS1). Black 1 (ASS1). Black circles (ACTB), tubulin tubulin (TUBA1A), and arginine succinate synthase circles represent proteins represent proteins absent from the list of interactors fed by the user for the software input list. Asterisks absent in the list of interactors fed by the user to the computer software input list. Asterisks indicate protein indicate protein interactions manually added for the PPI network in line with the N-Butanoyl-DL-homoserine lactone References literature (MAP7, interactions manually added to the PPI network in line with the literature (MAP7, RAI14, Homer2). RAI14, Homer2). Striped circles denote the four CX26 interactor candidates Emetine Cancer previously demonstrated Striped circles denote the 4 CX26 interactor candidates previously demonstrated to associate with to associate with other CX. Thicker lines indicate experimentally demonstrated associations that may other CX. Thicker lines indicate experimentally demonstrated associations that may be either more be either much more direct interaction. A dashed line indicates dashed of an intermediate interactor. reproducible or possibly a reproducible or a direct interaction.
The PPI network includes all 12 proteins classified as cell junction/cytoskeleton (Table 1, Figure 1B) and one from the mitochondrion group (argininosuccinate synthase 1ASS1, Figure 1A), which has been reported to interact with the plasma membrane inInt. J. Mol. Sci. 2018, 19, 2535 Int. J. Mol. Sci. 2018, 19, x FOR PEER REVIEW4 of 20 4 ofaddition for the mitochondrial outer membrane [28]. Within the present study, we report the analysis of membrane [28]. In the present study, we report the evaluation of your group of 13 proteins present within the the group of 13 proteins present within the PPI network. PPI network.2.two. Identified CX Binding Partners inside the CX26 Molecular Complicated two.two. Identified CX Binding Partners in.
