Nd caspase-8 in monocytes and mouse intestinal epithelial cells [25,26], and results of a study indicating that IL-10 alters the c-FLIP/caspase balance in human dermal fibroblasts [27]. In addition, in our study results showed that IL-10 treatment mainly significantly down-regulated activecaspase-3, it may demonstrated that IL-10 may mainly regulate apoptosis of trophoblast cells induced by T. gondii infection via affecting the caspase3-active-caspase3 pathway. Most of the apoptosis observed at 24 hr post-infection occurred in uninfected cells in the vicinity of T. gondii nfected cells as evidenced by apoptosis in nuclei at a distance from parasitophorous vacuoles. This observation is consistent with other reports [18,28,29]. This “bystander killing” occurs in trophoblast cultures infected with cytomegalovirus [30] in which infected cells release or express agents cytotoxic to 23727046 neighboring cells. In this study, we found that IL-10 reduced the extent of apoptosis of uninfected cells in the vicinity of T. gondii infected cells at early infection stage (24 hr), but the detailed mechanisms involved as well as the effects on pregnancy of infected mice deserve further investigation.AcknowledgmentsWe thank Professor Striepen of the Tropical and Emerging Global Diseases Center, Georgia University, USA, for the kind gift of YFP-T. gondii and Professor Zheng Jing of Chinese Medicine Hospital, Yantai, P. R. China, for providing human tissues.Author ContributionsConceived and designed the experiments: XH MZ RZ XX. Performed the experiments: XH MZ RZ XX YL HZ XZ. Analyzed the data: XH MZ RZ HZ. Contributed reagents/materials/analysis tools: XH MZ RZ XX YL XZ. Wrote the paper: XH MZ RZ XX. Collected samples: RZ.
Type 2 diabetes affects over 300 million people worldwide, with the incidence of the disease expected to reach over 500 million by 2030 [1]. Insulin resistance and high blood glucose levels characterize the disease but its causes are multi-factorial [2,3]. One of the Title Loaded From File hallmarks of advanced type 2 diabetes is the development of amyloid plaques consisting of the endocrine hormone Title Loaded From File amylin (also known as islet amyloid polypeptide or IAPP) [4]. The amyloid plaques have been implicated in the destruction of pancreatic b-cells that synthesize both amylin and insulin [3,4]. As with other amyloid diseases it is unclear whether fibrils or soluble oligomers are responsible for amylin pathology [5?]. Even if fibrils are not the main culprits, their properties are important to understand since they could serve as a reservoir from which toxic oligomers dissociate [9]. The structure of amylin fibrils has been characterized by solidstate nuclear magnetic resonance (ssNMR) [10], electron paramagnetic resonance (EPR) [11], two-dimensional infrared spectroscopy (2DIR) [12] and cryo-electron microscopy (cryo-EM) [10,11,13]. The consensus from these studies is that the amylin monomers adopt a hairpin structure composed of two b-strands in the fibrils. Each of the b-strands forms an intermolecular parallel b-sheet pairing with the equivalent b-strand from an adjacent amylin monomer. Two stacks of b-hairpins related by C2symmetry run in opposite directions along the length of the fibril and pack against each other to form the protofilament building block of the fibrils [10]. As with other amyloid fibrils, more subtle aspects of the structure are less clear and show larger differencesbetween models obtained by different techniques. These include the precise sequence l.Nd caspase-8 in monocytes and mouse intestinal epithelial cells [25,26], and results of a study indicating that IL-10 alters the c-FLIP/caspase balance in human dermal fibroblasts [27]. In addition, in our study results showed that IL-10 treatment mainly significantly down-regulated activecaspase-3, it may demonstrated that IL-10 may mainly regulate apoptosis of trophoblast cells induced by T. gondii infection via affecting the caspase3-active-caspase3 pathway. Most of the apoptosis observed at 24 hr post-infection occurred in uninfected cells in the vicinity of T. gondii nfected cells as evidenced by apoptosis in nuclei at a distance from parasitophorous vacuoles. This observation is consistent with other reports [18,28,29]. This “bystander killing” occurs in trophoblast cultures infected with cytomegalovirus [30] in which infected cells release or express agents cytotoxic to 23727046 neighboring cells. In this study, we found that IL-10 reduced the extent of apoptosis of uninfected cells in the vicinity of T. gondii infected cells at early infection stage (24 hr), but the detailed mechanisms involved as well as the effects on pregnancy of infected mice deserve further investigation.AcknowledgmentsWe thank Professor Striepen of the Tropical and Emerging Global Diseases Center, Georgia University, USA, for the kind gift of YFP-T. gondii and Professor Zheng Jing of Chinese Medicine Hospital, Yantai, P. R. China, for providing human tissues.Author ContributionsConceived and designed the experiments: XH MZ RZ XX. Performed the experiments: XH MZ RZ XX YL HZ XZ. Analyzed the data: XH MZ RZ HZ. Contributed reagents/materials/analysis tools: XH MZ RZ XX YL XZ. Wrote the paper: XH MZ RZ XX. Collected samples: RZ.
Type 2 diabetes affects over 300 million people worldwide, with the incidence of the disease expected to reach over 500 million by 2030 [1]. Insulin resistance and high blood glucose levels characterize the disease but its causes are multi-factorial [2,3]. One of the hallmarks of advanced type 2 diabetes is the development of amyloid plaques consisting of the endocrine hormone amylin (also known as islet amyloid polypeptide or IAPP) [4]. The amyloid plaques have been implicated in the destruction of pancreatic b-cells that synthesize both amylin and insulin [3,4]. As with other amyloid diseases it is unclear whether fibrils or soluble oligomers are responsible for amylin pathology [5?]. Even if fibrils are not the main culprits, their properties are important to understand since they could serve as a reservoir from which toxic oligomers dissociate [9]. The structure of amylin fibrils has been characterized by solidstate nuclear magnetic resonance (ssNMR) [10], electron paramagnetic resonance (EPR) [11], two-dimensional infrared spectroscopy (2DIR) [12] and cryo-electron microscopy (cryo-EM) [10,11,13]. The consensus from these studies is that the amylin monomers adopt a hairpin structure composed of two b-strands in the fibrils. Each of the b-strands forms an intermolecular parallel b-sheet pairing with the equivalent b-strand from an adjacent amylin monomer. Two stacks of b-hairpins related by C2symmetry run in opposite directions along the length of the fibril and pack against each other to form the protofilament building block of the fibrils [10]. As with other amyloid fibrils, more subtle aspects of the structure are less clear and show larger differencesbetween models obtained by different techniques. These include the precise sequence l.
