In LVEDP and the reduction in +dP/dt was attenuated in animals subjected to 8 weeks of exercise training. Analysis of Oxidative Stress 
by the Dihydroethidium Fluorescence Analysis of superoxide formation showed a Cilomilast significant increase in the fluorescence of OVX+MISED when compared with other groups. However, in the OVX+MIET group,Exercise training CEP32496 reduced left ventricular end diastolic pressure in OVX+MIET group compared to MI which occurs in parallel with an increased +dP/dt. There were a decrease in -dP/dt in the MI group compared with other groups which was not restored by ET. Data are expressed as mean SEM. P,0.05. doi:10.1371/journal.pone.0115970.g002 9 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 3. Analysis of oxidative stress in cardiac tissue. Analysis of superoxide formation in sections of cardiac tissue by the dihydroethidium fluorescence. Representative images of Control, OVX+SHAMSED, OVX+SHAMET, OVX+MISED and OVX+MIET groups. Data are expressed as mean SEM. P,0.05. Bar: 200 mm. doi:10.1371/journal.pone.0115970.g003 by MI, which demonstrates the efficacy of ET in the maintenance of ROS homeostasis. Protein Expression The expression of pro-oxidant and antioxidant enzymes was verified in order to analyze the possible mechanisms involved in the preservation of cardiac function. The expression of the AT1 receptor was significantly reduced in the OVX+MIET group compared with the OVX+MISED group. There was a significant increase in Gp91phox protein expression in MI rats compared to control group. On the other hand, ET was able to significantly reduce the expression of Gp91phox in MI rats. No significant differences in SOD-2 expression 10 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 4. Oxidant and antioxidants PubMed ID:http://jpet.aspetjournals.org/content/120/2/255 proteins expression. Expression of AT1 receptor, Gp91phox and the antioxidant enzymes 
SOD-2 and catalase. The densities were normalized to the control protein GAPDH. Data are expressed as mean SEM. P,0.05. doi:10.1371/journal.pone.0115970.g004 were found. However, a significant increase was observed in catalase expression in the OVX+SHAMET compared to OVX+MISED and OVX+SHAMSED and in the OVX+MIET group compared with Control, OVX+SHAMSED and OVX+MISED rats. Evaluation of Interstitial Collagen and Myocyte Hypertrophy The myocardial area occupied by collagen was evaluated in transverse sections of LV stained with Picrosirius. The images were represented in Fig. 5. A significant increase in collagen deposition was observed in MI rats compared with the Control, OVX+SHAMSED and OVX+SHAMEF groups. 11 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 5. Interstitial collagen deposition evaluation in rat hearts. Representative images of histological sections stained with Picrosirius of Control, OVX+SHAMSED, OVX+SHAMET, OVX+MISED and OVX+MIET groups. Data are expressed as mean SEM. P,0.05. Magnifier 400x. Bar: 200 mm. doi:10.1371/journal.pone.0115970.g005 However, exercise training reduced myocardial collagen deposition after MI. Fig. 6 shows representative histological sections stained with hematoxylin and eosin. After MI, a significant increase in myocyte cross sectional area was observed when compared to OVX+SHAMSED and OVX+SHAMET groups. However, ET in MI rats was able to prevent the myocyte hypertrophy. Discussion Many studies have investigated ET as a strategy to modify the remodeling process after MI. Therefore, the aim of this study was analyze the role of ET in the prevention or attenuation of car.In LVEDP and the reduction in +dP/dt was attenuated in animals subjected to 8 weeks of exercise training. Analysis of Oxidative Stress by the Dihydroethidium Fluorescence Analysis of superoxide formation showed a significant increase in the fluorescence of OVX+MISED when compared with other groups. However, in the OVX+MIET group,Exercise training reduced left ventricular end diastolic pressure in OVX+MIET group compared to MI which occurs in parallel with an increased +dP/dt. There were a decrease in -dP/dt in the MI group compared with other groups which was not restored by ET. Data are expressed as mean SEM. P,0.05. doi:10.1371/journal.pone.0115970.g002 9 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 3. Analysis of oxidative stress in cardiac tissue. Analysis of superoxide formation in sections of cardiac tissue by the dihydroethidium fluorescence. Representative images of Control, OVX+SHAMSED, OVX+SHAMET, OVX+MISED and OVX+MIET groups. Data are expressed as mean SEM. P,0.05. Bar: 200 mm. doi:10.1371/journal.pone.0115970.g003 by MI, which demonstrates the efficacy of ET in the maintenance of ROS homeostasis. Protein Expression The expression of pro-oxidant and antioxidant enzymes was verified in order to analyze the possible mechanisms involved in the preservation of cardiac function. The expression of the AT1 receptor was significantly reduced in the OVX+MIET group compared with the OVX+MISED group. There was a significant increase in Gp91phox protein expression in MI rats compared to control group. On the other hand, ET was able to significantly reduce the expression of Gp91phox in MI rats. No significant differences in SOD-2 expression 10 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 4. Oxidant and antioxidants PubMed ID:http://jpet.aspetjournals.org/content/120/2/255 proteins expression. Expression of AT1 receptor, Gp91phox and the antioxidant enzymes SOD-2 and catalase. The densities were normalized to the control protein GAPDH. Data are expressed as mean SEM. P,0.05. doi:10.1371/journal.pone.0115970.g004 were found. However, a significant increase was observed in catalase expression in the OVX+SHAMET compared to OVX+MISED and OVX+SHAMSED and in the OVX+MIET group compared with Control, OVX+SHAMSED and OVX+MISED rats. Evaluation of Interstitial Collagen and Myocyte Hypertrophy The myocardial area occupied by collagen was evaluated in transverse sections of LV stained with Picrosirius. The images were represented in Fig. 5. A significant increase in collagen deposition was observed in MI rats compared with the Control, OVX+SHAMSED and OVX+SHAMEF groups. 11 / 18 Exercise and Myocardial Infarction in OVX Rats Fig. 5. Interstitial collagen deposition evaluation in rat hearts. Representative images of histological sections stained with Picrosirius of Control, OVX+SHAMSED, OVX+SHAMET, OVX+MISED and OVX+MIET groups. Data are expressed as mean SEM. P,0.05. Magnifier 400x. Bar: 200 mm. doi:10.1371/journal.pone.0115970.g005 However, exercise training reduced myocardial collagen deposition after MI. Fig. 6 shows representative histological sections stained with hematoxylin and eosin. After MI, a significant increase in myocyte cross sectional area was observed when compared to OVX+SHAMSED and OVX+SHAMET groups. However, ET in MI rats was able to prevent the myocyte hypertrophy. Discussion Many studies have investigated ET as a strategy to modify the remodeling process after MI. Therefore, the aim of this study was analyze the role of ET in the prevention or attenuation of car.
