S and Tuberculosistuberculin skin test 1516647 or the IFNc release assays [10]. Therefore, latent infection is thought to reflect a critical balance between Th1 and Th17 responses that serve to control the pathogen and Th2 cells, regulatory T cells and immunoregulatory cytokines (e.g.,IL10 and TGFb) that limit immune-mediated pathology [9]. Apart from 
latent infection, a common form of less severe TB disease is TB lymphadenitis [2], a form thought to be associated with extrapulmonary spread through a hematogenous or a lymphatic route. To study roles of T cell cytokines and potential regulatory factors, we examined Mtb antigen-specific induction of Type 1, 2, and 17 responses as well as production of IL-10 and TGFb in pulmonary TB (PTB), tuberculous lymphadenitis (TBL) and latent TB (LTB) individuals in an area highly endemic for tuberculosis. We observed that active pulmonary TB was characterized by a dimunition of 
spontaneous and antigen-specific production of Type 1, 2 and 17 cytokines. TBL individuals, in contrast to those with PTB, exhibited a reduction only in the production of Type 1 (but not Type-2 or -17) cytokines. The suppression of cytokine responses in PTB was primarily mediated by IL-10.Hematologic analysisHematology was performed on all patients using the Act-5 Diff hematology analyzer (Beckman Coulter). As shown in Table 1, there were no significant differences in the baseline hematological parameters between PTB, TBL and LTB individuals.SMER 28 manufacturer AntigensMycobacterial antigens – PPD (Statens Serum Institute, Copenhagen, Denmark), ESAT-6 and CFP–10 (both recombinant proteins from Fitzgerald Industries Intl. Inc) were used as antigenic stimuli, and anti-CD3 antibody was used as positive control. Final concentrations were 10 mg/ml for PPD, ESAT-6 and CFP-10 and 5 mg/ml for anti-CD3.In vitro cultureWhole blood cell cultures were performed to determine the levels of cytokines. Briefly, whole blood was diluted 1:1 with RPMI-1640 medium supplemented with penicillin/streptomycin (100 U/100 mg/ml), L-glutamine (2 mM), and HEPES (10 mM) (all from Invitrogen) and distributed in 12-well tissue culture plates (Costar, Corning Inc). The cultures were then stimulated with PPD, ESAT-6, CFP-10, or anti-CD3 or media alone. After 72 h, culture supernatants were collected and analyzed for cytokines. For cytokine neutralization experiments, whole blood was cultured in the presence of anti-IL-10 (5 mg/ml), anti-TGFb (5 mg/ml) or isotype control antibody (5 mg/ml) (all from R 15755315 D Sytems) for 1 h following which PPD was added and cultured for a further 72 h.Methods Study populationWe studied a group of 71 individuals; 26 with PTB, 23 with TBL and 22 individuals with LTB (Table 1). Individuals with PTB were diagnosed by positive sputum acid-fast bacillus (AFB) ZiehlNeelsen staining and solid cultures in Lowenstein – Jensen medium. Individuals with TBL were diagnosed on the basis of clinical examination and AFB staining and culture of fine-needle AN-3199 site aspiration biopsies of lymph nodes. Individuals were diagnosed as having LTB on the basis of being positive in the Quantiferon-TB Gold in Tube (Cellestis) assay but having an absence of pulmonary symptoms concurrent with a normal chest radiograph. All subjects had been bacillus Calmette-Guerin (BCG) vaccinated at birth. All ?the individuals were HIV negative and blood was collected prior to commencement of anti-TB treatment. This clinical protocol was approved by the Institutional Review Board of the National Institute of.S and Tuberculosistuberculin skin test 1516647 or the IFNc release assays [10]. Therefore, latent infection is thought to reflect a critical balance between Th1 and Th17 responses that serve to control the pathogen and Th2 cells, regulatory T cells and immunoregulatory cytokines (e.g.,IL10 and TGFb) that limit immune-mediated pathology [9]. Apart from latent infection, a common form of less severe TB disease is TB lymphadenitis [2], a form thought to be associated with extrapulmonary spread through a hematogenous or a lymphatic route. To study roles of T cell cytokines and potential regulatory factors, we examined Mtb antigen-specific induction of Type 1, 2, and 17 responses as well as production of IL-10 and TGFb in pulmonary TB (PTB), tuberculous lymphadenitis (TBL) and latent TB (LTB) individuals in an area highly endemic for tuberculosis. We observed that active pulmonary TB was characterized by a dimunition of spontaneous and antigen-specific production of Type 1, 2 and 17 cytokines. TBL individuals, in contrast to those with PTB, exhibited a reduction only in the production of Type 1 (but not Type-2 or -17) cytokines. The suppression of cytokine responses in PTB was primarily mediated by IL-10.Hematologic analysisHematology was performed on all patients using the Act-5 Diff hematology analyzer (Beckman Coulter). As shown in Table 1, there were no significant differences in the baseline hematological parameters between PTB, TBL and LTB individuals.AntigensMycobacterial antigens – PPD (Statens Serum Institute, Copenhagen, Denmark), ESAT-6 and CFP–10 (both recombinant proteins from Fitzgerald Industries Intl. Inc) were used as antigenic stimuli, and anti-CD3 antibody was used as positive control. Final concentrations were 10 mg/ml for PPD, ESAT-6 and CFP-10 and 5 mg/ml for anti-CD3.In vitro cultureWhole blood cell cultures were performed to determine the levels of cytokines. Briefly, whole blood was diluted 1:1 with RPMI-1640 medium supplemented with penicillin/streptomycin (100 U/100 mg/ml), L-glutamine (2 mM), and HEPES (10 mM) (all from Invitrogen) and distributed in 12-well tissue culture plates (Costar, Corning Inc). The cultures were then stimulated with PPD, ESAT-6, CFP-10, or anti-CD3 or media alone. After 72 h, culture supernatants were collected and analyzed for cytokines. For cytokine neutralization experiments, whole blood was cultured in the presence of anti-IL-10 (5 mg/ml), anti-TGFb (5 mg/ml) or isotype control antibody (5 mg/ml) (all from R 15755315 D Sytems) for 1 h following which PPD was added and cultured for a further 72 h.Methods Study populationWe studied a group of 71 individuals; 26 with PTB, 23 with TBL and 22 individuals with LTB (Table 1). Individuals with PTB were diagnosed by positive sputum acid-fast bacillus (AFB) ZiehlNeelsen staining and solid cultures in Lowenstein – Jensen medium. Individuals with TBL were diagnosed on the basis of clinical examination and AFB staining and culture of fine-needle aspiration biopsies of lymph nodes. Individuals were diagnosed as having LTB on the basis of being positive in the Quantiferon-TB Gold in Tube (Cellestis) assay but having an absence of pulmonary symptoms concurrent with a normal chest radiograph. All subjects had been bacillus Calmette-Guerin (BCG) vaccinated at birth. All ?the individuals were HIV negative and blood was collected prior to commencement of anti-TB treatment. This clinical protocol was approved by the Institutional Review Board of the National Institute of.
